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Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron
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Dissection and Immunohistochemistry of the Drosophila Adult Leg to Detect Changes at the Neuromuscular Junction for an Identified Motor Neuron

Article DOI: 10.3791/62844-v • 11:02 min • February 12th, 2022
February 12th, 2022 •
1, *1,2
1Department of Biology, Rhode Island College, 2Department of Pediatrics, Women and Infants Hospital, Warren Alpert Medical School of Brown University
* These authors contributed equally

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We describe a dissection technique that preserves the architecture of the neuromuscular junction and enables a detailed immunocytochemical study of motor neurons in the adult Drosophila leg.

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Dissection Immunohistochemistry Drosophila Adult Leg Neuromuscular Junction Motor Neuron Cuticle Removal Tissue Exposure Neuronal Architecture Muscle Architecture Immunocytic Chemical Techniques Slow Degenerative Changes Larval Neuromuscular Junction Stability Adult Neuron Lifespan Genotypes Disease Models Antibodies Paint Brush Transfer
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