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Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells

Article DOI: 10.3791/62902-v • 06:17 min • August 13th, 2021
August 13th, 2021 •
1,2, 1,2, 1, 1, *2,3, *1
1Department of Physiology, Faculty of Medicine in Hradec Králové, Charles University, 2Department of Pathophysiology, Third Faculty of Medicine, Charles University, 3Department of Internal Medicine, University Hospital Kralovske Vinohrady
* These authors contributed equally

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In this work, we describe a modified protocol to test mitochondrial respiratory substrate flux using recombinant perfringolysin O in combination with microplate-based respirometry. With this protocol, we show how metformin affects mitochondrial respiration of two different tumor cell lines.

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Mitochondrial Substrate Flux Recombinant Perfringolysin O Permeabilized Cells Cancer Cell Response Metformin Treatment Research Use Only Mitochondrial Metabolism Warriors Drugs Affecting Mitochondria Karolina Vaneckova Undergraduate Student Research Technician A549 Cells Seahorse XF 96 Cell Culture Micro-plate Cell Attachment Experimental Group Control Group Sterile Distilled Water Sensor Cartridge
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