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Isolation og kultur af primære menneskelige gingivale epitelceller ved hjælp af Y-27632
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1Department of Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine,Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 2Department of Orthodontics, School and Hospital of Stomatology,Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 3Department of Stomatology,Shengli Oilfield Central Hospital, 4Department of Pediatric Surgery,Shengli Oilfield Central Hospital, 5Ningbo Stomatology Hospital of Savaid Stomatology School,Hangzhou Medical College
Chapitres
- 00:00Introduction
- 00:49Gingival Tissue Pretreatment
- 01:13Digestion of Gingival Tissue
- 02:46Cell Passage
- 03:51Representative Results
- 06:18Conclusion
Her præsenterer vi en modificeret metode til isolering og kultur af menneskelige gingivale epitelceller ved at tilføje Rock-hæmmeren, Y-27632, til den traditionelle metode. Denne metode er lettere, mindre tidskrævende, forbedrer stamcelleegenskaberne og producerer et større antal højpotentialede epitelceller både til laboratoriet og til kliniske anvendelser.
