Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Characterization of Neuromuscular Junctions in…
JoVE Journal
Neuroscience

Ce contenu est disponible gratuitement.

JoVE Journal Neuroscience
Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy
Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina
Journal (Neuroscience)
Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina
Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue
Journal (Neuroscience)
Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue
 

Characterization of Neuromuscular Junctions in Mice by Combined Confocal and Super-Resolution Microscopy

Article DOI: 10.3791/63032-v • 11:03 min • December 8th, 2021
December 8th, 2021 •
*1,2, *1, 1,2, 1,2, *1,3, *1,2
1Genethon, 91000, Evry, France, 2Université Paris-Saclay, Univ Evry, Inserm, Genethon, Integrare research unit UMR_S951, 91000, Evry, France, 3Ecole Pratique des Hautes Etudes, PSL Research University, 75014, Paris, France
* These authors contributed equally

Chapitres

résumé
Automatic Translation

This protocol describes a method for morphometric analysis of neuromuscular junctions by combined confocal and STED microscopy that is used to quantify pathological changes in mouse models of SMA and ColQ-related CMS.

Tags

Neuromuscular Junctions Mice Confocal Microscopy Super-resolution Microscopy Protocol 3D Structure Electron Microscopy Muscle Samples Immunolabeling Morphological Quantification Subcellular Structures Muscle Teasing Immunostainings Image Acquisition Image Analysis Tibialis Anterior Muscles Fiber Bundles Triton X-100 PBS Blocking Solution BSA
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter