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Use of Crystal Violet to Improve Visual Cytopathic Effect-based Reading for Viral Titration using TCID50 Assays

Article DOI: 10.3791/63063-v • 04:06 min • February 12th, 2022
February 12th, 2022 •
1, 1
1College of Veterinary Medicine, Department of Population Health and Pathobiology, North Carolina State University

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This protocol shows an accurate and objective approach to visualize viral titrations using crystal violet, by comparing it with optical microscopy and immunocytochemical staining.

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Crystal Violet Visual Cytopathic Effect Viral Titration TCID50 Assays Virus Stock Staining 96-well Plate Biosafety Level 2 Laboratory Cell Line Dilutions Vortex Media PBS Inoculum Incubation PFA Fixation
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