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Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex
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Neurosciences
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Journal JoVE Neurosciences
Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex

Ex Vivo Optogenetic Interrogation of Long-Range Synaptic Transmission and Plasticity from Medial Prefrontal Cortex to Lateral Entorhinal Cortex

DOI:
10.3791/63077-v

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11:31 min

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February 25, 2022

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,
1School of Physiology, Pharmacology and Neuroscience,University of Bristol

Chapitres

  • 00:04Introduction
  • 00:45Stereotaxic Viral Injection
  • 02:32Preparation of Acute Brain Slices
  • 04:14Electrophysiology and Optogenetic Stimulation
  • 06:57Histology
  • 09:25Results: Viral Transduction of Discrete Brain Regions with Optogenetic Constructs
  • 10:41Conclusion

Summary

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Here we present a protocol describing viral transduction of discrete brain regions with optogenetic constructs to permit synapse-specific electrophysiological characterization in acute rodent brain slices.

Tags

OptogeneticsSynaptic TransmissionSynaptic PlasticityMedial Prefrontal CortexLateral Entorhinal CortexVirally Delivered ConstructsAcute Brain SlicesLong-range PathwaysSelective StimulationStereotaxic InjectionViral PreparationBrain DissectionSucrose Cutting Solution

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