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Controlled Semi-Automated Laser-Induced Injuries for Studying Spinal Cord Regeneration in Zebrafish Larvae

Article DOI: 10.3791/63259-v • 09:43 min • November 22nd, 2021
November 22nd, 2021 •
1, 1, 1, 1, 1,2, 1,2
1Centre for Discovery Brain Sciences, University of Edinburgh Medical School: Biomedical Sciences, 2Center for Regenerative Therapies at the TU Dresden

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The present protocol describes a method to induce tissue-specific and highly reproducible injuries in zebrafish larvae using a laser lesion system combined with an automated microfluidic platform for larvae handling.

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Controlled Semi-automated Laser-induced Injuries Spinal Cord Regeneration Zebrafish Larvae Manual Lesions Protocols Rotating Glass Capillary Targeted UV Laser VAST Equipment Anesthetizing Larvae 96-well Plate Automated Zebrafish Imaging VAST Software Capillary Air Bubbles LP Sampler Window Script File Experiment File
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