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Purification of Endogenous Drosophila Transient Receptor Potential Channels

Article DOI: 10.3791/63260-v • 08:39 min • December 28th, 2021
December 28th, 2021 •
*1, *1, 1, 1, 1, 1
1Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center
* These authors contributed equally

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Based on the assembling mechanism of the INAD protein complex, in this protocol, a modified affinity purification plus competition strategy was developed to purify the endogenous Drosophila TRP channel.

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Purification Endogenous Drosophila TRP Channels Protocol Purified Full-length TRP Channels Electrophysiological Properties Structural Information INAD Protein Complex Nickel Beads Fly Heads Yuyang Liu Technician PGEX 4T-1 TRP 1261-1265 Plasmid E.coli BL21 Cells Calcium Chloride Heat Shock Transformation Method Luria Bertani Medium Seeding Culture Optical Density Isopropyl-beta-D-1-thiogalactopyranoside Overexpression Centrifugation Phosphate-buffered Saline High Pressure Homogenizer Circularly Pass
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