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Isolering og karakterisering af intakt Phycobilisome i cyanobakterier
Isolation and Characterization of Cyanobacterial Extracellular Vesicles
Journal (Biology)
Isolation and Characterization of Cyanobacterial Extracellular Vesicles
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Journal (Biology)
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Isolering og karakterisering af intakt Phycobilisome i cyanobakterier

Article DOI: 10.3791/63272-v • 06:26 min • November 10th, 2021
November 10th, 2021 •
1, 1,2
1Department of Life Science, National Taiwan University, 2Institute of Plant Biology, National Taiwan University

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Den nuværende protokol beskriver isoleringen af fykobiliomer fra cyanobakterier ved centrifugering gennem en diskontinuerlig saccharosetæthedsgradient. Fraktioner af intakte phycobilisomes bekræftes af 77K fluorescerende emissionsspektrum og SDS-PAGE analyse. De resulterende fykobilisome fraktioner er velegnede til negativ farvning af TEM og massespektrometri analyse.

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Biologi Udgave 177 Cyanobacterium fycobilisome Synechocystis sp. PCC 6803 Leptolyngbya sp. JSC-1 perleskægter homogenizer diskontinuerlig saccharosetæthedsgradient 77K fluorescens emissionsspektrum
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