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JoVE Journal Developmental Biology
Isolatie van rat vetweefsel Mesenchymale stamcellen voor differentiatie in insulineproducerende cellen
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Isolatie van rat vetweefsel Mesenchymale stamcellen voor differentiatie in insulineproducerende cellen

Article DOI: 10.3791/63348-v • 08:09 min • August 29th, 2022
August 29th, 2022 •
1, 2, 2, 1,2,3
1Biochemistry Department, Faculty of Pharmacy, Ain Shams University, 2Pharmacology and Biochemistry Department, Faculty of Pharmacy, The British University in Egypt, 3Center for Drug Research and Development (CDRD), Faculty of Pharmacy, The British University in Egypt

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Vetweefsel-afgeleide mesenchymale stamcellen (Ad-MSC's) kunnen een potentiële bron zijn van MSC's die differentiëren in insulineproducerende cellen (IPC's). In dit protocol bieden we gedetailleerde stappen voor de isolatie en karakterisering van epididymale Ad-MSC's bij ratten, gevolgd door een eenvoudig, kort protocol voor het genereren van IPC's van dezelfde rat Ad-MSC's.

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Ontwikkelingsbiologie Nummer 186 Stamcellen mesenchymale stamcellen diabetes insulineproducerende cellen vetweefsel differentiatie
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