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Comparison of Methods for Isolating Entomopathogenic Fungi from Soil Samples

Article DOI: 10.3791/63353-v • 07:16 min • January 6th, 2022
January 6th, 2022 •
1, 2, 3, 2, 1,3, 1,3
1Programa de Pós-Graduação em Ciências Veterinárias, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, 2Laboratório de Taxonomia Bioquímica e Bioprospecção de Fungos/Coleção de Cultura de Fungos Filamentosos, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz (FIOCRUZ), 3Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro

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résumé
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Entomopathogenic fungal colonies are isolated from tropical soil samples using Tenebrio bait, Galleria bait, as well as selective artificial medium, i.e., potato dextrose agar enriched with yeast extract supplemented with chloramphenicol, thiabendazole, and cycloheximide (CTC medium).

Tags

Entomopathogenic Fungi Isolation Biopesticide Development Insect Bait Method Chloramphenicol Thiabendazole Cycloheximide Artificial Media CTC Plates Macro Morphology Micro Morphology Soil Sample Collection Rhizosphere Competence Selective Artificial Medium Biosafety Cabinet
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