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Subretinal Implantation of RPE on a Carrier in Minipigs: Guidelines for Preoperative Preparations, Surgical Techniques, and Postoperative Care

Article DOI: 10.3791/63505-v • 11:06 min • November 11th, 2022
November 11th, 2022 •
*1,2, *3, 4, 4, 4, 5,6, 5,6, 5,6, 5,6, 5,6, 5, 5, 5, 5, 7, 8, 9, 10, 11, 1, 1, 5, *8, *5
1Eye Clinic, Department of Ophthalmology, University Hospital Giessen and Marburg GmbH, 2Karl Landsteiner Institute for Retinal Research and Imaging, 3Department of Ophthalmology, University Hospital Kralovske Vinohrady and Third Faculty of Medicine, Charles University in Prague, 4Institute of Macromolecular Chemistry, Czech Academy of Sciences, 5Institute of Animal Physiology and Genetics, Czech Academy of Sciences, 6Department of Cell Biology, Faculty of Science, Charles University, 7Institute of Experimental Medicine, Czech Academy of Sciences, 8Center for Eye Research, Department of Ophthalmology, Oslo University Hospital and Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, 9Moorfields Eye Hospitals UAE, 10Stem Cell Therapies in Neurodegenerative Diseases Lab, Research Center “Principe Felipe”, 11Eye Center Donaustadt, Department of Ophthalmology, Sigmund Freud University
* These authors contributed equally

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résumé
Automatic Translation

The subretinal implantation of retinal pigmented epithelium (RPE) is one of the most promising approaches for the treatment of degenerative retinal diseases. However, the performance of preclinical studies on large-eye animal models remains challenging. This report presents guidelines for the subretinal transplantation of RPE on a cell carrier into minipigs.

Tags

Subretinal Implantation RPE Carrier Minipigs Guidelines Preoperative Preparations Surgical Techniques Postoperative Care Age-related Macular Degeneration Central Vision Loss Progressive Degeneration Dysfunction Therapy For Retinal Diseases Treatment Modality In Vitro Cultured RPE Cells Cell Suspension Self-supporting Cell Sheet Cell Monolayer Artificial Carrier Differentiated Monolayer State Pre-clinical Animal Studies Surgical Procedure Large Eye Animal Model In Vivo Subretinal Implantation Experimental Studies Different Types Of Cells And Cell Carriers
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