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JoVE Journal Bioengineering
Amplifikation af Escherichia coli i en kontinuerlig-flow-PCR mikrofluidisk chip og dens påvisning med et kapillærelektroforesesystem
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Amplifikation af Escherichia coli i en kontinuerlig-flow-PCR mikrofluidisk chip og dens påvisning med et kapillærelektroforesesystem

Article DOI: 10.3791/63523-v • 14:12 min • November 21st, 2023
November 21st, 2023 •
1, 1, 1, 2, 1, 1, 1,2
1Shanghai Key Lab of Modern Optical System, University of Shanghai for Science and Technology, 2Graduate School of Engineering, Osaka University

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Denne protokol beskriver, hvordan man bygger et kontinuerligt flowpolymerasekædesystem baseret på en mikrofluidisk chip, og hvordan man bygger et kapillærelektroforesesystem i laboratoriet. Det præsenterer en enkel metode til analyse af nukleinsyrer i laboratoriet.

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