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Engineering Antiviral Agents via Surface Plasmon Resonance

Article DOI: 10.3791/63541-v • 13:00 min • June 14th, 2022
June 14th, 2022 •
1,2
1Department of Environmental Health Sciences, University of California, Los Angeles, 2Department of General Surgery, Medical University of Vienna

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The present protocol describes new tools for SPR binding assays to examine CV-N binding to HA, S glycoprotein, related hybrid-type glycans, and high-mannose oligosaccharides. SPR is used to determine the KD for binding either dimeric or monomeric CV-N to these glycans.

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Engineering Antiviral Agents Surface Plasmon Resonance Spectroscopy Protein Ligand Medium Throughput Binding Site Variants Iron-binding High Manners Oligosaccharides Viruses Label-free Technique Macromolecular Binding Surface Immobilized Receptors Multisite Interactions Kinetic Analysis Competitive Binding Specific Areas In Research Drug Development Inhibitors Antibody Characterization Kinetic Constants Affinities Sensor Response LB-agar Plates Double-stranded DNA Template Primer Concentration DpnI Restriction Enzyme Mineral Oil Overlay Incubate Supercompetent Cells
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