Ultrasound-Guided Induced Pluripotent Stem Cell-Derived Cardiomyocyte Implantation in Myocardial Infarcted Mice

Xun Wu; Kele Qin; Di Wang; Kun Xiang; Jun Peng; Jianjun Guo; Jinfu Yang; Chengming Fan

doi:10.3791/63647

Implantation de cardiomyocytes induite par ultrasons dérivée de cellules souches pluripotentes ch...

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Ultrasound-Guided Induced Pluripotent Stem Cell-Derived Cardiomyocyte Implantation in Myocardial Infarcted Mice

Implantation de cardiomyocytes induite par ultrasons dérivée de cellules souches pluripotentes chez des souris infarctus du myocarde

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08:03 min

March 30, 2022

DOI: 10.3791/63647-v

Xun Wu¹, Kele Qin¹, Di Wang², Kun Xiang¹, Jun Peng², Jianjun Guo³, Jinfu Yang^1,2, Chengming Fan^1,2,3

¹Department of Cardiovascular Surgery, Second Xiangya Hospital,Central South University, ²Hunan Provincial Key Laboratory of Cardiovascular Research, ³Hunan Fangsheng Pharmaceutical Co., Ltd.

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Overview

This study presents a novel approach for delivering human iPS-derived cardiomyocytes via echocardiography-guided, percutaneous intramyocardial injection. This method is shown to be safe, effective, and less invasive for treating myocardial infarction in mice.

Key Study Components

Area of Science

Cardiovascular research
Cell transplantation
Myocardial infarction treatment

Background

Myocardial infarction leads to significant heart damage.
Cell therapy using iPS-derived cardiomyocytes has potential for heart repair.
Current delivery methods may be invasive and less effective.
This study explores a less invasive delivery technique.

Purpose of Study

To evaluate the effectiveness of echocardiography-guided cell delivery.
To assess the safety of percutaneous intramyocardial injection.
To enhance the reparative properties of human iPS-derived cardiomyocytes.

Methods Used

Echocardiography-guided percutaneous injection technique.
Use of human iPS-derived cardiomyocytes.
Evaluation of cardiac function post-injection.
Comparison with traditional delivery methods.

Main Results

The method significantly enhances the reparative properties of cardiomyocytes.
It is a feasible and repeatable procedure.
Demonstrated safety in the context of myocardial infarction.
Potential application for other heart diseases.

Conclusions

Echocardiography-guided delivery is a promising technique for cell therapy.
It offers a less invasive alternative for myocardial infarction treatment.
Further research is warranted to explore its full potential.

Frequently Asked Questions

What is the main advantage of this delivery method?

The main advantage is that it is less invasive compared to traditional methods, making it safer for patients.

Who supervised this research project?

The project was supervised by Professor Jinfu Yang and Professor Jun Peng.

What type of cells are being delivered in this study?

Human iPS-derived cardiomyocytes are being delivered to enhance heart repair.

Is this method repeatable?

Yes, the method has been shown to be repeatable and effective.

What diseases could benefit from this technique?

This technique could benefit diseases such as ischemic heart disease, myocardial infarction, and heart failure.

L’administration de cellules guidée par ultrasons autour du site de l’infarctus du myocarde chez la souris est un moyen sûr, efficace et pratique de transplantation cellulaire.

Il s’agit du premier rapport démontrant que l’injection intramyocardique percutanée de cardiomyocytes guidée par échocardiographie améliore significativement la propriété réparatrice des cardiomyocytes humains dérivés de l’iPS. Cette méthode est une procédure réalisable, satisfaisante, reproductible, moins invasive et efficace pour administrer un traitement des cardiomyocytes dérivés des iPS humaines. Nous croyons que l’administration intramyocardique percutanée est une méthode sûre et efficace pour l’administration locale de médicaments pour des maladies telles que les cardiopathies ischémiques, l’infarctus du myocarde et l’insuffisance cardiaque.

Ce projet est supervisé par le professeur Jinfu Yang et le professeur Jun Peng. Xun Wu, Kele Qin et Kun Xiang, MD PhDs de mon laboratoire et Di Wang, un PhD du Laboratoire provincial de recherche cardiovasculaire du Hunan. Pour commencer, aspirez le surnageant de culture à partir d’une culture de 60 jours de hiPSC-CM.

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