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Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

Article DOI: 10.3791/63891-v • 08:02 min • May 5th, 2022
May 5th, 2022 •
1, 1, 1, 1, 1
1Université Paris Cité, CNRS, Institut Jacques Monod

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We present protocols for simple actin filament microfluidic assays, in combination with fluorescence microscopy, that allow one to accurately monitor individual actin filaments in real-time while sequentially exposing them to different protein solutions.

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Microfluidics Fluorescence Microscopy Actin Filaments Actin-binding Proteins Assembly Dynamics Bundles Reactions Microfluidic Chamber PDMS Glass Cover Slip UV Exposure Air Bubbles Hot Plate
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