Measurement of Endothelium-Dependent Vasorelaxation in the Mouse Thoracic Aorta Using Tensometric Small Volume Chamber Myography

Brikena Gusek; Robert Folk; Tala Curry; Mitra Esfandiarei

doi:10.3791/63918

Mesure de la vasorelaxation dépendante de l’endothélium dans l’aorte thoracique de souris à l’aid...

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Measurement of Endothelium-Dependent Vasorelaxation in the Mouse Thoracic Aorta Using Tensometric Small Volume Chamber Myography

Mesure de la vasorelaxation dépendante de l’endothélium dans l’aorte thoracique de souris à l’aide de la myographie tensométrique à petit volume

Full Text

4,270 Views

09:47 min

August 12, 2022

DOI: 10.3791/63918-v

Brikena Gusek¹, Robert Folk¹, Tala Curry², Mitra Esfandiarei¹

¹Department of Biomedical Sciences, College of Graduate Studies,Midwestern University, ²Department of Child Health, College of Medicine-Phoenix,University of Arizona, Phoenix

Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This protocol illustrates the application of the tensometric myograph technique for assessing mouse aortic endothelial function under controlled physiological conditions. The method is recognized for its reliability and reproducibility in studying vascular reactivity.

Key Study Components

Research Area

Vascular biology
Endothelial function assessment
Microscopic analysis of blood vessels

Background

The importance of endothelial function in vascular biology.
The role of tensometric myography in vascular reactivity studies.
Challenges in isolating and assessing particular layers of blood vessels.

Methods Used

Tensometric myography as the primary technique
Mouse aorta as the biological model
Use of HEPES-PSS solution and high-potassium solution for experimentation

Main Results

Demonstrated reproducibility of the measurements.
Successful isolation and assessment of aortic segments' function.
Enhanced understanding of acetylcholine-induced vasorelaxation dynamics.

Conclusions

The study validates the tensometric myograph technique for endothelial function research.
This method can significantly advance insights into vascular health and diseases.

Frequently Asked Questions

What is the significance of the tensometric myograph technique?

The tensometric myograph technique allows for precise measurement of vascular reactivity, which is crucial for understanding endothelial function.

How do researchers ensure the reliability of measurements?

Reliability is ensured through rigorous experimental protocols and by maintaining controlled physiological conditions throughout the assay.

What are the advantages of using mouse aorta in this research?

The mouse aorta serves as an accessible and relevant model for studying vascular biology and endothelial functions.

Can this technique be applied to other blood vessels?

Yes, the tensometric myograph technique can be adapted for use with other small resistance and large conduit blood vessels.

What role do vasoconstrictors play in this experiment?

Vasoconstrictors are used to pre-contract the aortic segments, allowing researchers to study subsequent vasorelaxation responses to various agents.

Is the technique suitable for high-throughput screening?

While the technique is robust, its detailed nature may limit the pace of high-throughput screenings compared to other methods.

What precautions are taken during the myographic measurements?

Precautions include maintaining optimal temperatures, aeration of solutions, and careful handling of aortic segments to prevent tissue damage.

Le présent protocole décrit les concepts et l’application technique de la technique du myographe tensométrique utilisant un système myographique à chambres multiples dans l’évaluation expérimentale ex vivo de la fonction endothéliale aortique de la souris.

Nous démontrons la myographie isométrique ou tensométrique comme protocole de référence pour mesurer la réactivité des petits vaisseaux sanguins de résistance ou de grands conduits dans un état physiologique bien contrôlé. Les mesures sont hautement reproductibles et fiables. La technique est très utile pour permettre aux chercheurs d’étudier différentes couches de vaisseaux sanguins séparément ou d’étudier simultanément l’interaction entre différentes couches de la paroi des vaisseaux sanguins.

Robert Folk, le spécialiste du laboratoire de recherche de mon laboratoire, fera la démonstration de la procédure. Commencez par allumer le bain-marie et réglez-le à 37 degrés Celsius. Placez deux béchers étiquetés de manière appropriée dans le bain-marie, l’un avec 600 millilitres de solution HEPES-PSS et l’autre avec 150 millilitres de solution riche en potassium.

View the full transcript and gain access to thousands of scientific videos

Explore More Videos

Biologie numéro 186 Myographie tensométrique à petite chambre aorte de souris fonction endothéliale vasorelaxation contraction vasculaire des muscles lisses