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Measurement of Endothelium-Dependent Vasorelaxation in the Mouse Thoracic Aorta Using Tensometric Small Volume Chamber Myography

Article DOI: 10.3791/63918-v • 09:47 min • August 12th, 2022
August 12th, 2022 •
1, 1, 2, 1
1Department of Biomedical Sciences, College of Graduate Studies, Midwestern University, 2Department of Child Health, College of Medicine-Phoenix, University of Arizona, Phoenix

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The present protocol describes the concepts and technical application of the tensometric myograph technique using a multi-chamber myograph system in the experimental ex vivo assessment of mouse aortic endothelial function.

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Endothelium-dependent Vasorelaxation Mouse Thoracic Aorta Tensometric Small Volume Chamber Myography Isometric Myography Gold Standard Protocol Reactivity Of Blood Vessels Small Resistance Blood Vessels Large Conduit Blood Vessels Reproducibility Reliability Layers Of Blood Vessels Blood Vessel Wall Interaction Water Bath HEPES-PSS Solution High-potassium Solution Carbogen Gas Centrifuge Tube Aortic Dissection Process Four-chamber Myograph Unit
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