Isolement et analyse de vésicules extracellulaires traçables et fonctionnalisées à partir du plasma et des tissus solides
2.6K vues
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Cité par 7
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09:57 min
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17 octobre 2022
DOI :10.3791/63990-v
17 octobre 2022
•2.6K vues
Yuan Cao*1,2 , Ji-Yu Qiu*1,3 , Da Chen1,2 , Chen-Yao Li1,4 , Shu-Juan Xing1,5 , Chen-Xi Zheng1 , Xin Liu2 , Yan Jin1,6 , Bing-Dong Sui1
1State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi International Joint Research Center for Oral Diseases, Center for Tissue Engineering, School of Stomatology, The Fourth Military Medical University, 2Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, 3Department of VIP Dental Care, School of Stomatology, The Fourth Military Medical University, 4School of Basic Medicine, The Fourth Military Medical University, 5College of Life Science, Northwest University, 6Xi'an Institute of Tissue Engineering and Regenerative Medicine
* These authors contributed equally
Le présent protocole décrit une méthode pour extraire les vésicules extracellulaires du sang périphérique et des tissus solides avec profilage ultérieur des antigènes de surface et des cargaisons de protéines.
Chapters in this video
0:05
Introduction
1:20
Preparation of the Plasma and the Maxillary Bone Samples and Extraction of Extracellular Vesicles
2:50
Origin Analysis of Extracellular Vesicles
5:17
Protein Content Analysis of Extracellular Vesicles
8:06
Results: Profiling of Surface Antigens and Protein Cargos of Extracellular Vesicles Isolated from In Vivo Samples
9:13
Conclusion
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