Modèle de don chez les grands animaux après mort circulatoire et perfusion régionale normamère pour l’évaluation cardiaque

Overview

This protocol describes a large animal model of donation after circulatory death followed by thoracoabdominal normothermic regional perfusion that closely mimics clinical heart transplantation. This model has the potential to facilitate therapeutic studies and strategies in translational research.

Key Study Components

Area of Science

• Cardiac transplantation
• Translational research
• Large animal models

Background

• The model uses porcine subjects, which share anatomical and surgical similarities with human hearts.
• It aims to improve preclinical evaluation of cardiac transplantation techniques.
• Normothermic regional perfusion is a critical component of the protocol.
• This approach may enhance the understanding of heart transplantation outcomes.

Purpose of Study

• To develop a suitable preclinical model for cardiac transplantation.
• To evaluate potential strategies in translational research.
• To simulate clinical scenarios in heart transplantation.

Methods Used

• Midline incision on an anesthetized porcine model.
• Layer-by-layer division of subcutaneous fat using electrocautery.
• Retraction and sweeping of soft tissues from under the sternum.
• Implementation of thoracoabdominal normothermic regional perfusion.

Main Results

• The protocol successfully mimics clinical heart transplantation.
• It provides a robust framework for evaluating cardiac transplantation techniques.
• Demonstrates the feasibility of using large animal models in research.
• Facilitates the study of therapeutic strategies in cardiac care.

Conclusions

• This model is advantageous for preclinical cardiac transplantation studies.
• It bridges the gap between experimental research and clinical application.
• Further studies could enhance understanding of heart transplantation outcomes.

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