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Live Imaging of Microtubule Dynamics in Glioblastoma Cells Invading the Zebrafish Brain

Article DOI: 10.3791/64093-v • 09:29 min • July 29th, 2022
July 29th, 2022 •
1, 1, 1
1Cell Polarity, Migration and Cancer Unit, Université Paris-Cité, Institut Pasteur, Équipe Labellisée Ligue Contre le Cancer

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We report a technique permitting live imaging of microtubule dynamics in glioblastoma (GBM) cells invading a vertebrate brain tissue. Coupling the orthotopic injection of fluorescently tagged GBM cells into a transparent zebrafish brain with high-resolution intravital imaging allows the measurement of cytoskeleton dynamics during in situ cancer invasion.

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Live Imaging Microtubule Dynamics Glioblastoma Cells Zebrafish Brain Brain Cancer Invasion Subcellular Intravital Imaging Spatial Temporal Resolution Microtubule Cytoskeleton In Vivo Models Microtubule Dynamics Analysis Protein Analysis Cell Culture Trypsin EDTA Centrifuge Tube Ice Cold PBS Transplantation
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