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JoVE Journal Neuroscience
Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation
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Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation

Article DOI: 10.3791/64187-v • 11:10 min • July 6th, 2022
July 6th, 2022 •
*1, *2, 3, 1
1Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, College of Medicine, Jinan University, 2Department of Clinical Pathology, First Affiliated Hospital of Jinan University, 3Program in Neuroscience, Department of Biomedical Sciences, College of Medicine, Florida State University
* These authors contributed equally

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résumé
Automatic Translation

Using in ovo electroporation, we devised a method to selectively transfect the auditory inner ear and cochlear nucleus in chicken embryos to achieve a cell-group-specific knockdown of fragile X mental retardation protein during discrete periods of circuit assembly.

Tags

Cell-autonomous Function Fragile X Mental Retardation Protein Auditory Circuit In Ovo Electroporation FMRP Knockdown Fragile X Syndrome Drug-inducible Vector System Small Hairpin RNA Gene Function Vestibular System Embryo Manipulation Neural Tube Transfection Otocyst Transfection Developmental Variations Viability Eggshell Marking Albumin Removal
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