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Immunology and Infection

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Flow Cytometry-Based Quantification and Analysis of Myocardial B-Cells

Article DOI: 10.3791/64344-v • 12:46 min • August 17th, 2022
August 17th, 2022 •
1, 1, 1
1Division of Cardiology, Department of Medicine, The Johns Hopkins University School of Medicine

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Here we report a protocol for the quantification and differentiation of myocardial B-lymphocytes based on their location in the intravascular or endothelial space using flow cytometry.

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Flow Cytometry Quantification Analysis Myocardial B-cells Perfusion Techniques Digestion Techniques Reproducible Flow Cytometry-based Analysis Recovery Yield Variability Flow Cytometry Counts Heart Samples Digestion And Isolation Method Extended Antibody Panel Immune Cell Types Organ Harvesting Retro-orbital Injection Euthanasia Skin Peeling Fascia Layer Chest Wall Cut Mediastinum Content
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