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A High-Throughput Platform for Culture and 3D Imaging of Organoids

Article DOI: 10.3791/64405-v • 07:42 min • October 14th, 2022
October 14th, 2022 •
1,2, 1, 1, 1, 1, 1, 1,3,4, 1,5
1Mechanobiology Institute (MBI), National University of Singapore, 2Biomedical Engineering Department, National University of Singapore, 3Department of Biological Sciences, National University of Singapore, 4IRL 3639 CNRS, 5Department of Microbiology & Immunology, Immunology Translational Research Program, Yong Loo Lin School of Medicine, National University of Singapore

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This paper presents a fabrication protocol for a new type of culture substrate with hundreds of microcontainers per mm2, in which organoids can be cultured and observed using high-resolution microscopy. The cell seeding and immunostaining protocols are also detailed.

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High-Throughput Platform Culture 3D Imaging Organoids Microtexture Cell Culture Dish Material Compatibility Microscopy Drug Screening Pipeline Cancer Research PDMS Mold UV Curable Adhesive UV Light Exposure Laboratory Procedures Optical Microscopy
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