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Cytotoxicity Assays with Zebrafish Cell Lines
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Cytotoxicity Assays with Zebrafish Cell Lines

Article DOI: 10.3791/64860-v • 08:22 min • January 6th, 2023
January 6th, 2023 •
1, 1, 1, 2, 2, 2, 1, 3, 2, 3
1Graduate Program in Genetics, Department of Genetics, Federal University of Paraná (UFPR), 2Safety of Product Department, Grupo Boticário, 3Department of Genetics, Federal University of Paraná (UFPR)

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This protocol presents commonly used cytotoxicity assays (Alamar Blue [AB], CFDA-AM, Neutral Red, and MTT assays) adapted for the assessment of cytotoxicity in zebrafish embryo (ZEM2S) and liver (ZFL) cell lines in 96-well plates.

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Environmental Sciences Acute Toxicity Test Concentrations Fish Assays Viability Endpoints Ecotoxicity Studies 96-well Plate Assay Fish Species Protocol Animal-based Studies Hepatocytes Embryonic Cells Plating Cells Cell Suspensions Culture Medium Multi-channel Pipette Polystyrene 96-well Plate
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