Isolation, Culture, and Adipogenic Induction of Stromal Vascular Fraction-derived Preadipocytes from Mouse Periaortic Adipose Tissue

Min Liang; Yijie Huang; Yangjing Jiang; Yunwen Hu; Zhaohua Cai; Ben He

doi:10.3791/65703

$Isolement, culture et induction adipogénique de préadipocytes dérivés de la fraction vasculaire s...$

JoVE Journal
Biology
Author Produced

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Isolation, Culture, and Adipogenic Induction of Stromal Vascular Fraction-derived Preadipocytes from Mouse Periaortic Adipose Tissue

Isolement, culture et induction adipogénique de préadipocytes dérivés de la fraction vasculaire stromale à partir de tissu adipeux périaortique de souris

Full Text

3,189 Views

06:56 min

July 21, 2023

DOI: 10.3791/65703-v

Min Liang*¹, Yijie Huang*¹, Yangjing Jiang¹, Yunwen Hu¹, Zhaohua Cai¹, Ben He¹

¹Department of Cardiology, Shanghai Chest Hospital,Shanghai Jiao Tong University School of Medicine

Please note that some of the translations on this page are AI generated. Click here for the English version.

Overview

This study addresses the standardized isolation, culture, and adipogenic induction of stromal vascular fraction-derived preadipocytes from mouse periaortic adipose tissue (PVAT). The findings provide insights into PVAT function and its interactions with vascular cells, paving the way for further exploration of adipogenesis and cellular communication.

Key Study Components

Research Area

Preadipocyte isolation and culture
Adipogenic induction
Perivascular adipose tissue (PVAT) function

Background

Challenges in PVAT availability and resource requirements
Significance of perivascular adipose tissue in vascular biology
Need for in vitro studies with primary preadipocytes

Methods Used

In vivo dissection and isolation techniques from mouse PVAT
Cell culture and adipogenic induction protocols
Microsurgery and enzymatic digestion methods

Main Results

Successful isolation of stromal vascular fraction from PVAT
Establishment of a robust culture system for preadipocytes
Framework for studying adipocyte-vascular cell interactions

Conclusions

This study provides a reliable method for isolating preadipocytes, facilitating research on PVAT.
The findings highlight the intricate relations between preadipocytes and vascular cells, contributing to greater understanding in biology research.

Frequently Asked Questions

What is perivascular adipose tissue?

Perivascular adipose tissue (PVAT) is the fat tissue surrounding blood vessels, playing a role in vascular function.

Why is the isolation of preadipocytes important?

Isolating preadipocytes allows researchers to study adipogenesis and interactions with other cell types in a controlled environment.

What are the challenges faced in isolating PVAT?

Challenges include limited availability of PVAT and the resource demands compared to using established cell lines.

How does adipogenic induction work?

Adipogenic induction involves stimulating preadipocytes to develop into mature adipocytes through specific growth factors and environmental conditions.

What can be studied using this isolation method?

This method enables studies on adipogenesis, the function of PVAT, and the signaling pathways involved in fat cell differentiation.

Are there any specific technologies used in this research?

Yes, technologies such as microscopy and enzymatic assays are utilized to analyze cells and their functions.

Ici, nous décrivons l’isolement, la culture et l’induction adipogénique de préadipocytes dérivés de la fraction vasculaire stromale à partir de tissu adipeux périaortique de souris, permettant l’étude de la fonction du tissu adipeux périvasculaire et de sa relation avec les cellules vasculaires.

Notre objectif est d’établir une approche standardisée pour la culture d’isolement et l’induction adipogénique de préadipocytes dérivés de la fraction vasculaire stromale à partir de PVAT de masse. Ce protocole nous permet d’approfondir nos recherches sur la fonction PVAT et sur la communication in vitro entre les adipocytes périaortiques et les cellules vasculaires. Les principaux défis auxquels nous sommes actuellement confrontés dans nos expériences comprennent la disponibilité limitée de la TVAP et les besoins en ressources humaines et matérielles supplémentaires par rapport à l’utilisation de préadipocytes immortalisés.

Notre protocole permet d’étudier la fonction du tissu adipeux périvasculaire et sa relation avec les cellules vasculaires. Nous offrons également une base pour l’étude de l’anneau et des gènes PVAT, ainsi que de l’hétérogénéité, et fournit une plate-forme pour explorer l’important régulateur de la différenciation PVAT et de l’adipogenèse in vitro.

View the full transcript and gain access to thousands of scientific videos

Explore More Videos