Induction of Acute Ischemic Stroke in Mice Using the Distal Middle Artery Occlusion Technique

Changlong Leng; Youwei Li; Yaojian Sun; Wei Liu

doi:10.3791/66134

Induction de l’AVC ischémique aigu chez la souris à l’aide de la technique d’occlusion de l’artèr...

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JoVE Journal Neuroscience

Induction of Acute Ischemic Stroke in Mice Using the Distal Middle Artery Occlusion Technique

Induction de l’AVC ischémique aigu chez la souris à l’aide de la technique d’occlusion de l’artère moyenne distale

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07:34 min

December 15, 2023

DOI: 10.3791/66134-v

Changlong Leng*¹, Youwei Li*¹, Yaojian Sun¹, Wei Liu¹

¹Wuhan Institute of Biomedical Sciences, School of Medicine,Jianghan University

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Overview

This study presents a detailed protocol for establishing a distal middle cerebral artery occlusion (dMCAO) model in C57BL/6J mice using transcranial electrocoagulation. It focuses on evaluating the resulting neurological behaviors and histopathological features observed post-occlusion.

Key Study Components

Area of Science

Neurological behavior assessment
Histopathological analysis
Ischemic stroke modeling

Background

The medial cerebral artery (MCA) is key in ischemic strokes in humans.
Various MCA occlusion methods exist, but dMCAO offers advantages in terms of surgical invasiveness and survival rates.
The model is beneficial for exploring long-term functional recovery.

Purpose of Study

To generate a reliable experimental ischemic stroke model in rodents.
To assess behavioral outcomes and histopathological changes post-stroke.
To utilize the model for future studies on cognition impairment.

Methods Used

Transcranial electrocoagulation was employed to induce dMCAO.
C57BL/6J mice were used to study neurological changes following occlusion.
Behavioral tests such as grip strength, pole test, adhesive removal, and cylinder test were utilized.
Histological examination of brain tissue was conducted post-behavioral assessments.

Main Results

Mice exhibited significant reductions in grip strength and increased descent times on various behavioral tests.
Histological analysis showed disordered neuron arrangements and reduced cell density in the peri-infarct area.

Conclusions

The study establishes a robust dMCAO model with high reproducibility for stroke research.
This methodology will enhance understanding of structural and behavioral outcomes after ischemic stroke.
Future applications could focus on cognitive impairments in post-stroke recovery.

Frequently Asked Questions

What are the advantages of using the dMCAO model?

The dMCAO model offers decreased surgical invasiveness, smaller infarction sizes, and improved survival rates, making it suitable for long-term studies.

How is the MCA occlusion implemented in this study?

The MCA occlusion is achieved via transcranial electrocoagulation, with careful monitoring of blood flow and subsequent closure of the surgical site.

What types of outcomes are measured in this research?

The study measures neurological behaviors through various tests and evaluates histopathological changes in the brain tissue after the occlusion.

How can this method be adapted for future research?

This methodology can be adapted for investigating various cognitive and sensory deficits after ischemic strokes using different behavioral paradigms.

What are the key limitations of this study?

Limitations include the model being solely conducted in rodents, which may not entirely replicate human pathology, and the focus on acute outcomes post-stroke.

What insights does this study offer about stroke recovery?

The findings provide insights into the structural and behavioral changes that occur after ischemic stroke, paving the way for future investigations into recovery mechanisms.

Ici, nous présentons un protocole pour établir un modèle distal d’occlusion de l’artère cérébrale moyenne (dMCAO) par électrocoagulation transcrânienne chez les souris C57BL/6J et évaluons le comportement neurologique et les caractéristiques histopathologiques ultérieurs.

Dans la présente recherche, nous visons à fournir un protocole complet et détaillé pour établir un modèle d’occlusion de l’artère cérébrale moyenne chez les souris C57BL/6J en utilisant l’électrocoagulation transcrânienne et nous avons évalué le comportement neurologique et les caractéristiques histopathologiques ultérieurs. L’artère cérébrale moyenne, ou ACM, est reconnue comme le principal site de l’AVC ischémique chez l’homme. Les méthodes d’occlusion MCA, y compris la technique, l’introduction photochimique et l’occlusion, ont été largement employées pour induire une ischémie cérébrale focale chez les rongeurs.

Comparé à d’autres modèles d’ischémie, ce modèle présente l’avantage d’un caractère moins invasif chirurgical, d’une taille d’infarctus plus petite et d’un taux de survie plus élevé, ce qui le rend plus adapté à l’étude de la récupération fonctionnelle à long terme après un accident vasculaire cérébral ischémique. Dans l’ensemble, l’approche actuelle génère efficacement un modèle expérimental fiable de l’AVC ischémique qui présente une grande capacité de survie et une excellente répétabilité. Par conséquent, cette méthodologie constitue un outil inestimable pour la recherche fondamentale et translationnelle dans le domaine de l’AVC.

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