Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models

Motoki Okamoto; Shohei Yamashita; Takumi Memida; Melanie Mendonca; Susanne Brueckner; Shin Nakamura; Satoru Shindo; Ria Achong-Bowe; Jeffrey Thompson; Xiaozhe Han; Toshihisa Kawai; Maiko Suzuki

doi:10.3791/66583

Mesures de microdureté sur les dents et l’os alvéolaire dans des modèles de maladies bucco-dentai...

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Microhardness Measurements on Tooth and Alveolar Bone in Rodent Oral Disease Models

Mesures de microdureté sur les dents et l’os alvéolaire dans des modèles de maladies bucco-dentaires chez les rongeurs

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06:16 min

April 26, 2024

DOI: 10.3791/66583-v

Motoki Okamoto*¹, Shohei Yamashita*¹, Takumi Memida¹, Melanie Mendonca¹, Susanne Brueckner¹, Shin Nakamura¹, Satoru Shindo¹, Ria Achong-Bowe¹, Jeffrey Thompson¹, Xiaozhe Han¹, Toshihisa Kawai¹, Maiko Suzuki¹

¹Department of Oral Science and Translational Research, College of Dental Medicine,Nova Southeastern University

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Overview

This study establishes a standardized protocol for microhardness analysis in tooth and alveolar bone, contributing to the understanding of hard tissue pathophysiology in rodent oral disease models, particularly dental fluorosis and ligature-induced periodontal bone resorption.

Key Study Components

Research Area

Microhardness testing in dental and bone tissue
Evaluation of environmental impacts on dental health
Pathophysiology of oral diseases

Background

Previous studies lacked detailed methodologies for microhardness testing
Importance of indentation site on microhardness values
Environmental factors like fluoride and PFAS influence dental pathology

Methods Used

Standardized sample preparation and polishing techniques
Rodent models for oral disease research
Utilization of microhardness testing with Vickers indenter

Main Results

Indentation site affects microhardness across enamel layers and dentin
Fluoride treatment results in lower enamel microhardness
Alveolar bone microhardness is significantly reduced in diseased models

Conclusions

The proposed protocol enhances the reliability of microhardness measurements in dental tissues
Findings underscore the need for standardized approaches in biological research assessing dental health

Frequently Asked Questions

What is microhardness testing?

Microhardness testing measures the hardness of materials at a microscopic scale, often used for evaluating dental and bone tissues.

Why is indentation site important in microhardness testing?

Different indentation sites within the enamel and dentin layers can yield varying microhardness values, affecting study results.

How does fluoride affect dental tissues?

Fluoride treatment can lead to reduced enamel microhardness and may compromise overall dental health.

What are PFAS, and why are they significant in this study?

PFAS are manmade chemicals that can impact dental and bone health, making them relevant for evaluating their mechanical properties in studies.

What is the significance of establishing a standardized protocol?

A standardized protocol ensures consistency and reliability in microhardness testing, facilitating meaningful comparisons among studies.

How can this research advance dental pathology studies?

It provides a methodology for better understanding how environmental factors influence oral health, particularly in disease models.

What are the implications of this research?

The findings could aid in developing effective prevention and treatment strategies for dental diseases associated with environmental exposures.

La microdureté est une propriété mécanique et un paramètre informatif pour l’évaluation de la physiopathologie des tissus durs. Ici, nous démontrons un protocole standardisé (préparation d’échantillons, polissage, surface plane et sites d’indentation) pour l’analyse de la microdureté dans les dents et l’os alvéolaire dans des modèles de maladies bucco-dentaires de rongeurs, à savoir la fluorose dentaire et la résorption osseuse parodontale induite par la ligature.

Dans cette recherche, nous nous sommes concentrés sur l’établissement d’essais de microdureté précis et cohérents. L’essai de microdureté est une méthode traditionnelle, mais n’ayez crainte, la méthodologie n’est pas suffisamment décrite dans les publications précédentes. Comme nous l’avons démontré dans le résultat, fournir une réduction minimale immédiate de la microdureté dépend des sites d’intention de l’intermédiaire, comme les couches intérieures, intermédiaires et externes.

Nos résultats suggèrent que le site d’intention est important pour obtenir des résultats précis et cohérents. La lacune scientifique est que les études précédentes n’ont pas suffisamment décrit les méthodes d’essai de microdureté, en particulier les sites de variation. Pour cette raison, les résultats de microdureté n’étaient pas cohérents entre les études.

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