Miniscope Recording Calcium Signals at Hippocampus of Mice Navigating an Odor Plume

Fabio M. Simoes de Souza; Ryan Williamson; Connor McCullough; Alec Teel; Gregory Futia; Ming Ma; Aaron True; John P. Crimaldi; Emily Gibson; Diego Restrepo

doi:10.3791/67039

Miniscope enregistrant des signaux calciques à l’hippocampe de souris naviguant dans un panache d...

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JoVE Journal Neuroscience

Miniscope Recording Calcium Signals at Hippocampus of Mice Navigating an Odor Plume

Miniscope enregistrant des signaux calciques à l’hippocampe de souris naviguant dans un panache d’odeurs

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06:21 min

September 20, 2024

DOI: 10.3791/67039-v

Fabio M. Simoes de Souza¹, Ryan Williamson², Connor McCullough³, Alec Teel¹, Gregory Futia³, Ming Ma¹, Aaron True⁴, John P. Crimaldi⁴, Emily Gibson^3,5, Diego Restrepo^1,5

¹Department of Cell and Developmental Biology,University of Colorado Anschutz Medical Campus, ²Neurotechnology Center,University of Colorado Anschutz Medical Campus, ³Department of Bioengineering,University of Colorado Anschutz Medical Campus, ⁴Civil, Environmental & Architectural Engineering,University of Colorado, ⁵Neuroscience Graduate Program,University of Colorado Anschutz Medical Campus

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Overview

This study investigates the brain-behavior relationship in the hippocampal CA1 region of mice as they navigate an odor plume. Using a combination of behavioral training and miniscope GCaMP6f recordings, the research aims to decode how hippocampal neurons process olfactory and spatial information, particularly in relation to Alzheimer's disease.

Key Study Components

Area of Science

Neuroscience
Behavioral neuroscience
Imaging techniques

Background

Understanding how neural circuits facilitate complex behaviors.
The role of hippocampal CA1 neurons in spatial navigation and olfactory processing.
The impact of Alzheimer's disease on hippocampal functionality.
Use of miniscope technology for in vivo neuronal recordings.

Purpose of Study

To investigate how CA1 hippocampal neurons contribute to odor-navigation behaviors.
To examine how these behaviors are altered in a mouse model of Alzheimer's disease.
To utilize advanced imaging techniques to correlate neural activity with behavior.

Methods Used

Miniscope recording of dorsal CA1 neurons during odor navigation tasks.
Mouse model of Alzheimer's disease for behavioral training and testing.
Setup included a chamber designed for odor plume navigation.
Automated software for odor delivery and behavioral monitoring.
Synchronization of video and neural recordings for data analysis.

Main Results

Neural activity correlated with behavioral events during odor navigation.
Calcium transients observed in dorsal CA1 were linked to various phases of the navigation task.
Ability to decode the spatial trajectory of mice based on CA1 calcium signals.
The study revealed important insights into the neural mechanisms underlying olfactory and spatial processing.

Conclusions

This research enhances understanding of hippocampal functions in navigation and odor processing.
Results contribute to insights on the impairment of these functions in Alzheimer's disease models.
Highlights the utility of mini scope technology in exploring neuronal functions related to complex behavior.

Frequently Asked Questions

What are the advantages of using miniscope technology?

Miniscope technology allows for real-time in vivo recordings of neuronal activity in freely moving animals, providing insights into behavioral correlates of neural dynamics.

How is the mouse model of Alzheimer's disease implemented in this study?

The study utilizes a genetically modified mouse model to examine impaired navigational behaviors and neuronal function in the context of Alzheimer's disease.

What types of data are obtained from this method?

Data includes neuronal calcium signals during behavioral tasks, which are analyzed to understand the relationship between neuronal activity and spatial navigation.

How can this method be adapted for other studies?

The protocols for setting up the odor navigation task and neuronal recording can be adapted to study other brain regions or behavioral paradigms based on odor processing.

What are the key limitations of this study?

Potential limitations include the specificity of the model to investigate only certain aspects of navigation and the reliance on genetic modifications that may not fully replicate human Alzheimer's disease.

Ce protocole étudie la relation cerveau-comportement dans l’hippocampe CA1 chez des souris naviguant dans un panache d’odeurs. Nous fournissons un protocole étape par étape, y compris la chirurgie pour accéder à l’imagerie de l’hippocampe, l’entraînement comportemental, l’enregistrement et le traitement du cerveau par miniscope GCaMP6f, et des données comportementales pour décoder la position de la souris à partir de l’activité neuronale du retour sur investissement.

Mes recherches visent à comprendre comment le cerveau traite les informations olfactives et spatiales. Et nous essayons de comprendre le rôle des neurones CA1 de l’hippocampe dans la navigation du panache d’odeurs. À l’heure actuelle, la technologie d’enregistrement libre des neurones à l’aide de microscopes miniatures a été utilisée pour faire avancer la recherche dans le domaine.

Nous avons constaté qu’il est possible de décoder la trajectoire de la souris naviguant dans le panache olfactif en fonction des neurones et des signaux calciques dans le CA1 dorsal. Cette technique combine les avantages de la technologie des mini-scopes pour l’enregistrement des signaux calciques GCaMP avec la rangée bien établie de navigation spatiale de l’hippocampe CA1. Pour mieux comprendre comment les circuits neuronaux entraînent des comportements complexes, nous étudierons comment la navigation du panache d’odeurs est altérée dans un modèle murin de la maladie d’Alzheimer avec une fonction anormale de l’hippocampe CA1.

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