Mesure de quatre sous-types de cellules NK utérines à l’aide d’une coloration immunohistochimique fluorescente multiplexée chez des femmes présentant des échecs d’implantation répétés
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08:29 min
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25 octobre 2024
DOI :10.3791/67284-v
25 octobre 2024
•2.2K vues
Lu Chen1 , Loucia Kit Ying Chan1 , Stacey Chui Yiu Wong1 , Joshua Jing Xi Li2 , Jacqueline Pui Wah Chung1 , Chi Chiu Wang1,3,4,5 , Mingqing Li6,7,8 , Tao Zhang1
1Department of Obstetrics and Gynaecology, Faculty of Medicine, The Chinese University of Hong Kong, 2Department of Pathology, School of Clinical Medicine, The University of Hong Kong, Queen Mary Hospital, 3Reproduction and Development Laboratory, Li Ka Shing Institute of Health Sciences, The Chinese University of Hong Kong, 4School of Biomedical Sciences, Prince of Wales Hospital, The Chinese University of Hong Kong, 5Chinese University of Hong Kong - Sichuan University Joint Laboratory in Reproductive Medicine, The Chinese University of Hong Kong, 6Laboratory for Reproductive Immunology, Hospital of Obstetrics and Gynecology, Fudan University, 7NHC Key Lab of Reproduction Regulation, Shanghai Institute for Biomedical and Pharmaceutical Technologies, Fudan University, 8Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases, Hospital of Obstetrics and Gynecology, Fudan University
Cette étude présente une méthode pionnière pour quantifier les sous-ensembles de cellules tueuses naturelles utérines pendant la fenêtre d’implantation à l’aide de techniques avancées de coloration immunohistochimique par fluorescence multiplexée.
Chapters in this video
0:00
Introduction
1:35
Multiplex Immunohistochemistry for Uterine NK Cell Subtype Analysis in Endometrial Tissues
4:26
Image Acquisition After Multiplex Immuno‐Staining for Distinguishing uNK Cell Subtypes in Endometrial Tissues
5:51
Image Analysis to Characterize uNK Cell Subtypes in Early Pregnancy Using Multiplex Immunohistochemistry
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