Erratum: Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro

סיכום

An erratum was issued for: Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro. The Protocol section was updated.

תקציר

An erratum was issued for: Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro. The Protocol section was updated.

Step 2.6 of the Protocol was updated from:

Add 50 µL of 10 µg/mL FN coating solution to the outlet well of the top perfusion channel of every microfluidic unit (Figure 1b, well A3). Press the pipette tip against the side of the well for correct filling of the well without trapping air bubbles (see Figure 2b). The channel should fill, and the liquid should pin on the outlet (well C1) without filling the outlet well.

to:

Add 50 µL of 10 µg/mL FN coating solution to the outlet well of the top perfusion channel of every microfluidic unit (Figure 1b, well A3). Press the pipette tip against the side of the well for correct filling of the well without trapping air bubbles (see Figure 2b). The channel should fill, and the liquid should pin on the inlet (well A1) without filling the inlet well.

Step 3.7 of the Protocol was updated from:

Aspirate FN-coating solution from the perfusion inlet (well A1). Add 25 µL of basal medium in the inlet wells (well A1).

to:

Aspirate FN-coating solution from the perfusion outlet well (A3) and replace with 25 µL of basal medium in the outlet well (well A3).

פרוטוקול

An erratum was issued for: Standardized and Scalable Assay to Study Perfused 3D Angiogenic Sprouting of iPSC-derived Endothelial Cells In Vitro. The Protocol section was updated.

Step 2.6 of the Protocol was updated from:

Add 50 µL of 10 µg/mL FN coating solution to the outlet well of the top perfusion channel of every microfluidic unit (Figure 1b, well A3). Press the pipette tip against the side of the well for correct filling of the well without trapping air bubbles (see Figure 2b). The channel should fill, and the liquid should pin on the outlet (well C1) without filling the outlet well.

to:

Add 50 µL of 10 µg/mL FN coating solution to the outlet well of the top perfusion channel of every microfluidic unit (Figure 1b, well A3). Press the pipette tip against the side of the well for correct filling of the well without trapping air bubbles (see Figure 2b). The channel should fill, and the liquid should pin on the inlet (well A1) without filling the inlet well.

Step 3.7 of the Protocol was updated from:

Aspirate FN-coating solution from the perfusion inlet (well A1). Add 25 µL of basal medium in the inlet wells (well A1).

to:

Aspirate FN-coating solution from the perfusion outlet well (A3) and replace with 25 µL of basal medium in the outlet well (well A3).