Ceramic Bead Homogenization
Ceramic Bead Homogenization - Related Videos
06:51
Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay
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Cited by 4 •
2021
This protocol presents a technique for probing protein-protein interactions using glutathione-linked donor beads with GST-fused TPR-motif co-chaperones and acceptor beads coupled with an Hsp90-derived peptide. We have used this technique to screen small molecules to disrupt Hsp90-FKBP51 or Hsp90-FKBP52 interactions and identified potent and selective Hsp90-FKBP51 interaction inhibitors.
09:59
Ceramic-matrix Composite Materials and Their Bending Properties
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2023
Source: Sina Shahbazmohamadi and Peiman Shahbeigi-Roodposhti-Roodposhti, School of Engineering, University of Connecticut, Storrs, CT Bones are composites, made of a ceramic matrix and polymer fiber reinforcements. The ceramic contributes compressive strength, and the polymer provides tensile and flexural strength. By combining ceramic and polymer materials in different amounts, the body can create unique materials tailored for a specific application. As biomedical engineers, having the ability...
08:29
Multi-material Ceramic-Based Components – Additive Manufacturing of Black-and-white Zirconia Components by Thermoplastic 3D-Printing (CerAM - T3DP)
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Cited by 27 •
2019
Here we describe a protocol for additively manufacturing black-and-white zirconia components by Thermoplastic 3D-Printing (CerAM - T3DP) and co-sintering defect-free.
11:07
Synthesis of Non-uniformly Pr-doped SrTiO3 Ceramics and Their Thermoelectric Properties
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Cited by 4 •
2015
A protocol for the synthesis and processing of polycrystalline SrTiO3 ceramics doped non-uniformly with Pr is presented along with the investigation of their thermoelectric properties.
03:32
Amplified Luminescent Proximity Homogeneous Assay: A Bead-Based Proximity Assay to Screen Small Molecules Inhibiting Protein-Protein Interactions
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2025
In this video, we demonstrate the amplified luminescent proximity homogeneous assay—a bead-based proximity assay. It utilizes homogeneously-sized acceptor and donor beads, immobilized with two proteins that tend to interact to screen potential small molecules inhibiting protein interactions.
FAQs
Vigorous agitation drives repeated collisions between the ceramic beads and the biological sample. These impacts create localized mechanical stress, while movement of the beads and sample generates shear that further breaks cell walls and membranes. Together, the forces expose intracellular contents for subsequent recovery, making the approach useful when biological material is physically difficult to disrupt.
Hard ceramic beads provide the rigid surfaces needed to transmit strong collision forces into the sample. Buffer conditions contribute a different function: they help stabilize intracellular material after disruption and support efficient lysis. Maintaining both effective mechanical contact and suitable chemical conditions improves the likelihood that released DNA, RNA, proteins, or other biomolecules remain available for analysis.
The method is especially valuable for samples that resist simpler forms of disruption, including microorganisms and tissue. Their structural properties can make intracellular release challenging, so repeated bead-driven impact and shear provide a practical way to break the material apart. This broad sample compatibility supports workflows in microbiology, molecular biology, diagnostics, and general biomolecule extraction.
Disruption produces a lysed sample containing intracellular components that were previously enclosed by cell walls or membranes. The resulting material can then proceed to extraction or analysis, depending on the target biomolecule. Because the released contents may include DNA, RNA, proteins, or other components, the preparation step can support several downstream molecular biology workflows rather than a single analytical endpoint.
A typical workflow places the biological sample with hard ceramic beads in a tube, adds an appropriate buffer, and vigorously agitates the mixture to generate repeated impact and shear. After disruption, the prepared material is used for extraction of the desired biomolecule or for a related analysis. Closed-tube processing helps contain the sample during this sequence.
Its speed, scalability, and compatibility with closed tubes make the technique practical when many biological samples require consistent disruption. The approach can prepare microorganisms or tissue for DNA, RNA, protein, or other biomolecule analysis. Consequently, it fits diverse settings, including molecular biology and microbiology research, diagnostic workflows, and studies requiring reproducible sample preparation.