Kinetic Assay
Kinetic Assay - Related Videos
08:07
Enzyme Assays and Kinetics
0 Views •
2023
Enzyme kinetics describes the catalytic effects of enzymes, which are biomolecules that facilitate chemical reactions necessary for living organisms. Enzymes act on molecules, referred to as substrates, to form products. Enzyme kinetic parameters are determined via assays that directly or indirectly measure changes in substrate or product concentration over time. This video will cover the basic principles of enzyme kinetics (including rate equations) and kinetic models. The concepts governing...
02:52
Cellular Impedance-Based Survival Kinetics Assay to Assess Virus Survival
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2025
This video describes a cellular impedance-based technique to study the survival kinetics of viruses that induce cytopathic effects. The method measures the electrical impedance of virus-infected adherent cells pre-treated with saline.
03:07
Kinetic Molecular Theory: Molecular Velocities, Temperature, and Kinetic Energy
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2020
The kinetic molecular theory qualitatively explains the behaviors described by the various gas laws. The postulates of this theory may be applied in a more quantitative fashion to derive these individual laws. Collectively, the molecules in a sample of gas have average kinetic energy and average speed; but individually, they move at different speeds. Molecules frequently undergo elastic collisions in which the momentum is conserved. Since the colliding molecules are deflected off at different...
13:22
Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
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Cited by 15 •
2011
An adhesion frequency assay for measuring receptor-ligand interaction kinetics when both molecules are anchored on the surfaces of the interacting cells is described. This mechanically-based assay is exemplified using a micropipette-pressurized human red blood cell as adhesion sensor and integrin αLβ2 and intercellular adhesion molecule-1 as interacting receptors and ligands.
10:01
Kinetics of Addition Polymerization to Polydimethylsiloxane
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2023
Source: Kerry M. Dooley and Michael G. Benton, Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA Polymers are molecules consisting of many repeating monomer units that are chemically bonded into long chains. They exhibit a broad range of physical properties, which are affected by their chemical structure, molecular weight and degree of polymerization. The polymer industry manufactures thousands of raw materials used in a broad variety of commercial products.1,2 The...
FAQs
The assay tracks a measurable signal at defined intervals and relates its change to reaction progress. A signal that changes as substrate is consumed or product forms can be analyzed to estimate reaction rates. These time-dependent measurements help distinguish stronger or weaker activity and support characterization of catalytic efficiency rather than merely recording whether a reaction occurred.
These conditions can alter the behavior of the biological reaction and make rate comparisons difficult. Maintaining consistent temperature, pH, and reagent concentrations helps ensure that observed differences reflect the biological variable under investigation, such as an inhibitor, activator, or molecular component, rather than uncontrolled changes in the assay environment.
Comparing reaction rates with and without an inhibitor or activator shows how regulatory molecules affect biological activity. A reduced rate can indicate suppression of the measured reaction, whereas an increased rate can indicate stimulation. These comparisons help researchers investigate regulatory mechanisms and assess how molecular interactions influence enzyme activity or related processes.
Researchers select a measurable signal, such as absorbance or fluorescence, and establish the substrate, product, or other reaction component being monitored. They then control temperature, pH, and reagent concentrations, record measurements at defined time intervals, and analyze the resulting time-course data. This workflow produces reaction-rate information suitable for comparing experimental conditions.
Absorbance and fluorescence provide measurable signals that can change as substrate concentration decreases or product formation increases. Recording either signal repeatedly creates a time course for analysis. The most appropriate readout depends on which reaction change produces a detectable signal, allowing researchers to quantify activity and compare molecular responses under controlled conditions.
These assays are useful when researchers need to examine enzyme activity, metabolism, signaling, drug action, or molecular interactions. Because the measurements describe reaction rates over time, they can reveal how biological functions respond to inhibitors, activators, or other experimental conditions. In this way, kinetic data support studies of regulatory mechanisms and molecular activity.