Tissue Punch
Tissue Punch - Related Videos
04:37
Sampling Prostate Cancer Tissue for Biobanking: An MRI-guided Targeted Technique to Remove Biopsy Punches from Fresh Tumor Tissue
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2023
This video demonstrates an MRI data-based technique to target specific tumor-containing regions in prostate tissue for sampling. The collected biopsy specimens can be either stored for biobanking purposes or used for subsequent downstream analysis.
02:20
Generating an Excisional Skin Wound in a Mouse Model via Punch Biopsy
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2025
This video demonstrates a punch biopsy procedure to create a precise wound in a mouse model. The excision exposes the subcutaneous layer of the skin, providing a suitable site for studying immune responses to localized pathogen infection.
06:19
Skin Punch Biopsy Explant Culture for Derivation of Primary Human Fibroblasts
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Cited by 163 •
2013
The fibroblast explant culture protocol from human skin punch biopsies is a technically robust and simple way to derive skin cells within 4-8 weeks for banking of about 15-20 million cells at a low passage number.
07:39
Corneal and Limbal Alkali Injury Induction Using a Punch-Trephine Technique in a Mouse Model
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Cited by 7 •
2023
This protocol describes a method to induce an accurate and reproducible corneal and limbal alkali injury in a mouse model. The protocol is advantageous as it allows for an evenly distributed injury to the highly curved mouse cornea and limbus.
06:53
SpOT the Correct Tissue Every Time in Multi-tissue Blocks
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Cited by 5 •
2015
The purpose of the Specimen Orientation Tag (SpOT) is to function as an orientation tool to aid in individual tissue identification in multi-tissue paraffin blocks. These protocols demonstrate how it is constructed easily from common, low-cost histology materials and serves as a reliable visual marker in paraffin blocks and sections.
FAQs
The tool’s hollow, sharp edge concentrates force along a circular boundary. Pressing and rotating or advancing it shears tissue within that boundary, producing a defined specimen while leaving nearby material comparatively intact. This localized action allows researchers to examine the sampled region separately from adjacent tissue and supports measurements tied to a specific anatomical location.
Consistent size and placement improve comparisons between experimental groups because each specimen represents a similar tissue region. Standardization can reduce variation caused by sampling different areas or collecting unequal amounts of material. In biology studies, this supports more focused evaluation of tissue structure, gene expression, and localized responses associated with disease or other experimental conditions.
A Tissue Punch targets a restricted, defined region rather than treating an entire tissue area as one sample. Its localized collection preserves comparatively more surrounding material and helps connect measurements to a particular site. That distinction is useful when biological changes vary within a tissue and when researchers need focused rather than broadly averaged observations.
A researcher first identifies the tissue region to be sampled and positions the hollow, sharp-edged tool over that location. The tool is then pressed into the tissue while being rotated or advanced to shear out the specimen. After collection, the defined piece can be directed to histological, molecular, or cell-isolation workflows.
Collected specimens can be used for histological assessment, which examines tissue structure, or for molecular analysis of localized biological material. They may also support cell isolation and measurement of regional biological responses. The appropriate downstream use depends on the research question, but the standardized sample provides a focused starting material for each workflow.
This approach is useful when investigators need comparable specimens from defined tissue locations across experimental groups. It can support studies of tissue structure, gene expression, localized biological responses, and disease-related changes. By linking each sample to a specific region, the method helps researchers examine spatially focused effects rather than relying only on broader tissue measurements.