Homo Lumo

The HOMO-LUMO concept in chemistry describes the energy relationship between a molecule’s highest occupied molecular orbital (HOMO) and lowest unoccupied molecular orbital (LUMO), which helps explain electronic structure and reactivity. During an electronic transition, an electron can absorb energy and move from the HOMO to the LUMO; the energy gap between these orbitals influences the wavelength of absorbed light and the ease of excitation. Frontier molecular orbital analysis uses the HOMO as an electron-donor region and the LUMO as an electron-acceptor region to interpret chemical bonding, charge transfer, photochemical behavior, reaction pathways, and the properties of molecular materials.

Homo Lumo - Related Videos

Research

JoVE Journal - Chemistry

Using Polystyrene-block-poly(acrylic acid)-coated Metal Nanoparticles as Monomers for Their Homo- and Co-polymerization

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Cited by 1 •

2015

We report protocols for “polymerizing” various types of polymer-encapsulated metal nanoparticles into long chains of “homo-“ and “co-polymers”.

Fluorescence Fluctuation Spectroscopy to Study Protein Homo-Oligomerization

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2025

This video demonstrates the technique of fluorescence fluctuation spectroscopy (FFS) to study protein homo-oligomerization. Fluorescently-labeled proteins in a sample are dimerized using an agent when studying protein oligomerization using FFS. Using a confocal microscope, as the proteins move in and out of the small observation volume, the analysis of the fluctuations in the brightness of the fluorescent molecules is performed to determine the oligomeric state of the proteins.

Education

JoVE Lab Manual - Chemistry

UV-Vis Spectroscopy of Dyes - Concepts

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2020

Absorbance When light interacts with a substance, a portion of the light is absorbed while the rest is either reflected or transmitted through it. Substances that we perceive as having a color reflect light in the visible range. The color of the substance that we are able to see depends on which wavelength of light is reflected. A substance that we perceive as blue reflects light in the blue range (430 – 480 nm) of the visible spectrum. According to the color wheel, the same substance absorbs...

Chemical Inactivation of the E3 Ubiquitin Ligase Cereblon by Pomalidomide-based Homo-PROTACs

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Cited by 14 •

2019

This work describes the synthesis and characterization of a pomalidomide-based, bifunctional homo-PROTAC as a novel approach to induce ubiquitination and degradation of the E3 ubiquitin ligase cereblon (CRBN), the target of thalidomide analogs.

Generating Homo- and Heterografts Between Watermelon and Bottle Gourd for the Study of Cold-responsive MicroRNAs

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Cited by 6 •

2018

Here we present a detailed protocol for efficiently making homo- and heterografts between watermelon and bottle gourd, in addition to methods of tissue sampling, data generation, and data analysis, for the investigation of cold-responsive microRNAs.

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