Plant Tissue Extraction
Plant Tissue Extraction - Related Videos
05:43
Extraction of Genomic DNA from Plant Leaf Tissue Infected with Pathogenic Bacteria
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2025
Source: Gulla, S., et al. Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis. J. Vis. Exp. (2019).This protocol extracts genomic DNA from Yersinia ruckeri by suspending isolated colonies in ultrapure water and applying heat to lyse the cells. Centrifugation separates the DNA-containing supernatant from cell debris, yielding template DNA suitable for PCR-based genotyping and epidemiological analysis.
02:57
Plant Tissue Culture
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2020
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species. Plant tissue culture depends on the ability of plant tissue to give rise to an entire new plant when provided with a growth medium and appropriate environment. This ability of plant cells or tissues is termed ‘totipotency.’ The fundamental steps...
10:54
Extraction of Plant-based Capsules for Microencapsulation Applications
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Cited by 5 •
2016
This protocol/manuscript describes a streamlined process for the production of sporopollenin exine capsules (SECs) from Lycopodium clavatum spores and for the loading of hydrophilic compounds into these SECs.
08:51
Northern Blot to Detect and Quantify Specific MicroRNA in Total RNA Extract of Plant Tissue
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2025
In this video, we demonstrate the northern blot, a hybridization technique to detect and quantify microRNAs (miRNAs) from total RNA extracted from plant tissue.
10:37
Procedure to Evaluate the Efficiency of Flocculants for the Removal of Dispersed Particles from Plant Extracts
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Cited by 6 •
2016
The design-of-experiments procedure presented here allows the evaluation of different flocculants in terms of their ability to aggregate dispersed particles in plant extracts, thus reducing turbidity and the costs of downstream processing.
FAQs
Solvent polarity influences which soluble constituents transfer from disrupted plant material into the extract. Changing the solvent can therefore alter the balance of pigments, lipids, proteins, nucleic acids, and secondary metabolites recovered. Chemists use this effect to favor the compounds needed for identification, analysis, or later product development while reducing unwanted components.
These conditions influence how efficiently constituents move from plant tissue into the solvent. Particle size affects the material exposed to the solvent, contact time controls the duration of transfer, and temperature and pH can change extraction performance. Controlling these variables helps produce more consistent extracts for chemical analysis and comparison.
Cell disruption makes intracellular constituents more accessible to the selected solvent. Its importance depends on the target material, since plant extracts may contain pigments, lipids, proteins, nucleic acids, or secondary metabolites. Effective disruption supports transfer into the solvent, whereas insufficient access can reduce the amount available for subsequent filtration, identification, or analysis.
A basic workflow begins with plant material and a selected solvent, followed by disruption or maceration to promote contact between the tissue and solvent. The mixture is allowed to interact under controlled conditions, and filtration is then used as part of the preparation of the extract. The resulting material can undergo identification or chemical analysis.
The approach depends on the constituents of interest and the need to limit unwanted components. Chemists may use maceration, solvent extraction, and filtration in different combinations, while adjusting solvent polarity, temperature, particle size, contact time, or pH. These choices help tailor the resulting extract for phytochemical screening, quality control, or research.
Extracts provide material for identifying and analyzing plant constituents, including pigments, lipids, proteins, nucleic acids, and secondary metabolites. They support phytochemical screening, natural-product discovery, environmental analysis, and quality control. In applied settings, the same work contributes to developing pharmaceuticals, foods, cosmetics, and agricultural products from plant-derived chemical resources.