Luminescence Based Sensing
Luminescence Based Sensing - Related Videos
04:17
Approximate Number Sense Test
0 Views •
2023
Source: Laboratory of Jonathan Flombaum—Johns Hopkins University A common carnival game is to ask people to guess the number of jellybeans packed into a jar. The chances that anyone will get the exact number right are low. But what about the chances that someone will guess 17 or 147,000? Probably even less than the chances of guessing the correct answer; 17 and 147,000 just seem irrational. Why? After all, if the beans cannot be taken out and counted one-at-a-time, how can someone tell that an...
05:15
The Rouge Test: Searching for a Sense of Self
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2023
Source: Laboratories of Nicholaus Noles and Judith Danovitch—University of Louisville Humans are different from other animals in many ways, but one of the abilities that sets humans apart is their advanced ability to understand other people and simulate their thoughts and feelings, even when the thoughts and feelings do not align with their own. In scientific terms, these abilities are referred to as theory of mind, and this understanding is necessary for activities like giving compliments,...
02:14
Expression of the Luminescence Operon in Marine Bioluminescent Bacteria
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2025
Source: Brodl, E., et al. In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria. J. Vis. Exp. 1 (2018)This video demonstrates the cultivation of marine bioluminescent bacteria to observe quorum-sensing–mediated activation of the luminescence operon, enabling real-time monitoring of gene expression and light emission for studies in microbial communication and gene regulation.
04:00
Luminescence-Based Assay to Assess Neurotoxicity of Test Compounds on Neurons
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2025
In this video, we describe a luminescence-based method to assess the neurotoxicity of neurite outgrowth-promoting small molecule compounds on the viability of hNPC-derived neurons. The assay measures intracellular ATP level, an indicator of cell viability, to evaluate neurotoxicity following test compound treatment.
07:13
Nanostructured Ag-zeolite Composites as Luminescence-based Humidity Sensors
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Cited by 9 •
2016
A protocol for the synthesis of moisture-responsive luminescent Ag-zeolite composites is described in this report.
FAQs
A sensing interaction can modify several measurable properties of emitted light. Emission intensity indicates how much light is produced, wavelength identifies shifts in the color or spectral position, and lifetime captures how long emission persists. Examining one or more of these responses helps distinguish changes caused by an analyte or environmental condition and supports quantitative monitoring in engineering systems.
Quenching reduces the observed luminescence, whereas enhancement increases it when the sensing material interacts with a target or condition. Energy transfer provides another pathway for changing the emitted signal by coupling processes within the sensing system. These mechanisms convert chemical or physical interactions into optical responses that can be measured through changes in intensity, wavelength, or lifetime.
Calibration connects an optical response to the quantity being measured. A change in intensity, wavelength, or lifetime must be related to a known oxygen level, temperature, pressure, chemical concentration, or biological target before it can support quantification. This relationship allows engineers to interpret luminescent signals consistently and use them for monitoring rather than relying only on qualitative detection.
The workflow establishes how a selected luminescent response changes with the target variable. Depending on the application, calibration can relate emission intensity, wavelength, or lifetime to oxygen, temperature, pressure, chemical concentration, or a biological target. The resulting relationship provides the basis for converting measurements from the sensing material into information about conditions within an engineering system.
Remote measurements are valuable when the sensing location is difficult to access or when noncontact operation benefits an engineering system. Miniaturized implementations support compact devices and integrated sensing platforms, while retaining the ability to monitor chemical or physical conditions through optical responses. These features make the approach relevant to smart materials, distributed monitoring concepts, and compact engineering instrumentation.
The approach supports process monitoring, environmental assessment, and structural diagnostics, alongside development of smart materials and integrated sensing platforms. Its optical responses can provide information about oxygen, temperature, pressure, chemical concentrations, or biological targets. Consequently, engineers can apply the measurements to track operating conditions, assess environments, examine structural behavior, or incorporate sensing functions into advanced materials and devices.