Homozygous Mutant Animals
Homozygous Mutant Animals - Related Videos
10:07
Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
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Cited by 3 •
2022
This study provides a systematically optimized procedure of CRISPR/Cas9 ribonuclease-based construction of homozygous locust mutants as well as a detailed method for cryopreservation and resuscitation of the locust eggs.
08:39
Shifting Zebrafish Lethal Skeletal Mutant Penetrance by Progeny Testing
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Cited by 8 •
2017
The goal of this protocol is to alter the penetrance of lethal skeletal mutant phenotypes in zebrafish by selective breeding. Lethal mutants cannot be grown to adulthood and bred themselves, therefore this protocol describes a method for tracking and selecting penetrance through multiple generations by progeny testing.
09:17
Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish
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2025
Here, we describe a protocol for generating maternal mutant that couples a stable zpc:cas9 knock-in line with Tol2-mediated delivery of sgRNA expression cassettes.
11:02
Use of Arabidopsis eceriferum Mutants to Explore Plant Cuticle Biosynthesis
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Cited by 11 •
2008
The plant cuticle is a waxy outer covering on plants that has a primary role in water conservation but is also an important barrier against the entry of pathogenic microorganisms. In this video, we demonstrate the analysis of plant cuticle mutants identified by forward and reverse genetics approaches.
26:43
Computer-Generated Animal Model Stimuli
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Cited by 8 •
2007
Computer-generated stimuli using the Jacky dragon as a model.
FAQs
Having the altered allele on both homologous chromosomes can make a gene's effect more apparent than in a heterozygous animal, particularly when the phenotype is recessive. This arrangement lets investigators examine consequences associated with complete mutant pairing at that locus, then relate the observed phenotype to gene function while accounting for the animal's defined genetic background.
Wild-type animals provide a reference without the altered allele, heterozygous animals show the effect of one mutant copy, and homozygous mutants show the effect of two matching copies. Examining these groups together helps distinguish effects associated with the mutation from background variation and clarifies whether a trait follows a recessive pattern. This comparison strengthens genetic interpretation.
Homozygous mutant animals may be generated by inheritance or by genome editing, but the two routes frame interpretation differently. Inheritance links the genotype to parental transmission, whereas editing is described as introducing matching alterations into both homologous chromosomes. In either case, analysis benefits from a defined genetic background and comparisons with heterozygous and wild-type animals.
A breeding-based approach starts with carrier parents and focuses on offspring that inherit the altered allele from both sides. Those animals provide the homozygous group for subsequent phenotype analysis. Including the parental carrier state and comparison groups helps connect the observed trait to transmission of the allele rather than treating the phenotype as an isolated observation.
Genome-editing approaches are relevant when researchers want matching alterations introduced into both homologous chromosomes rather than relying solely on parental transmission. The resulting animals can support targeted examination of gene function in a defined genetic background. Their value is greatest when investigators also compare outcomes with heterozygous and wild-type animals, which provide context for the edited phenotype.
Beyond gene-function studies, these animals serve as models for human disease and for research on development, physiology, and potential therapies. Their phenotypes can reveal consequences of a specific genetic change in an organism, while inheritance-focused analysis can expose recessive patterns. Thus, the same model connects molecular alteration with organism-level traits and translational questions.