Spatial Localization
Spatial Localization - Related Videos
08:52
Spatial Molecular Imaging of the Glycome Using Mass Spectrometry
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Cited by 1 •
2025
This protocol details the key steps to enable rigorous and reproducible measurement of both glycogen and N-linked glycans using mass spectrometry imaging.
07:51
Spatial Cueing
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2023
Source: Laboratory of Jonathan Flombaum—Johns Hopkins University Attention refers to the limited human ability to select some information for processing at the expense of other stimuli in the environment. Attention operates in all sensory modalities: vision, hearing, touch, even taste and smell. It is most often studied in the visual domain though. A common way to study visual attention is with a spatial cueing paradigm. This paradigm allows researchers to measure the consequences of focusing...
08:44
Assessing Spatial Learning and Memory in Small Squamate Reptiles
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Cited by 3 •
2017
This paper describes a modification of the Barnes maze, a standard rodent paradigm used to assess spatial memory and learning, for use in small squamate reptiles.
10:16
Mining Spatial Transcriptomics Datasets using DeepSpaceDB
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2025
This article introduces a protocol for using DeepSpaceDB, a dynamic, interactive database for spatial transcriptomics, offering analysis workflows and examples to explore tissue organization and disease-related gene expression.
12:42
Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)
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Cited by 14 •
2015
Protein-protein interactions are visualized in cells with nanometer spatial resolution by combining bimolecular fluorescence complementation (BiFC) with photoactivated localization microscopy (PALM). Described here is the use of BiFC-PALM for imaging Ras-Raf interactions in U2OS cells for visualizing the nanoscale clustering and diffusion of individual Ras-Raf complexes.
FAQs
Chemokine gradients provide directional cues that guide immune-cell trafficking through tissues. Their distribution helps determine where immune cells accumulate in relation to infected or inflamed areas, bringing host defenses into proximity with microbes and activated cells. As a result, the location of immune-cell recruitment can influence whether local cellular interactions control infection or contribute to tissue damage.
Adhesion molecules help immune cells maintain interactions with particular tissue sites, while receptor signaling allows cells to respond to local molecular cues. Together, these mechanisms regulate where cells move, attach, and interact within defined compartments. Their activity therefore affects the organization of immune responses and helps determine which cells encounter pathogens or participate in inflammation.
Tissue barriers and cellular compartments restrict or channel the movement of immune cells, molecules, and pathogens. These boundaries can separate microbes from host defenses, concentrate interactions in particular regions, or support pathogen persistence within protected sites. Examining localization across such compartments helps explain why infection and immune activation may be unevenly distributed within the same tissue.
These approaches map where cells, molecules, and pathogens occur relative to one another rather than measuring only their overall abundance. The resulting spatial patterns can distinguish sites of infection, inflammation, and immune activation, while also revealing distributions associated with pathogen persistence. This information connects local organization with the tissue-level behavior of the immune response.
Studies can use imaging, microscopy, or spatial profiling to map distributions within defined tissues or cellular compartments. The selected approach records the locations of relevant cells, molecules, or pathogens and allows researchers to compare those patterns with infection, inflammation, or immune activation. Such mapping provides a spatial basis for interpreting local host-microbe interactions.
Therapeutic strategies can be informed by knowing where pathogens encounter host defenses, where immune activation occurs, and where microbes persist. Spatial information links local cellular interactions to broader outcomes, helping researchers consider interventions that target infection or limit tissue damage. It also provides context for evaluating how vaccines and anti-infective treatments influence tissue-level immune organization.