Crispr Gene Editing
Crispr Gene Editing - Related Videos
02:30
A CRISPR-Cas9 Technique for Gene Editing in T Cells
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2025
This video demonstrates an assay for performing gene editing in human T cells using the CRISPR-Cas9 technology. A mixture of primary CD4+ and CD8+ T cells is combined with a CRISPR-Cas9 ribonucleoprotein complex, targeting specific genes for knockout. Upon electroporation, the sgRNA guides Cas9 to the target DNA sequence, creating precise cuts. These cuts are then repaired by the cell's non-homologous end-joining mechanism, leading to gene knockout.
11:37
QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
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Cited by 5 •
2017
Details are presented on how QTL mapping with a whole genome sequence based genetic map can be used to identify a drug resistance gene in Toxoplasma gondii and how this can be verified with the CRISPR/Cas9 system that efficiently edits a genomic target, in this case the drug resistance gene.
04:22
A Technique for Gene Editing in Natural Killer Cells Using CRISPR Cas9
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2025
This video demonstrates a technique for Cas9 ribonucleoprotein-mediated genetic modification of primary natural killer (NK) cells. A Cas9 ribonucleoprotein, consisting of a Cas9 endonuclease bound to a guide RNA (gRNA) formed by base pairing a CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA), is introduced into primary natural killer cells via electroporation. The ribonucleoprotein targets and cleaves the host DNA at the target site, leading to gene knockout via modification of the...
16:08
Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing
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Cited by 34 •
2018
A simple and efficient microinjection protocol for gene editing in channel catfish embryos using the CRISPR/Cas9 system is presented. In this protocol, guide RNAs and Cas9 protein were microinjected into the yolk of one-cell embryos. This protocol has been validated by knocking out two channel catfish immune-related genes.
02:58
CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution
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2025
This video explains the concept of CRISPR-mediated cytosine base editors for inducing targeted nucleotide substitution.
FAQs
The cell’s response to the nuclease-created DNA break helps determine the editing result. Repair can disrupt the targeted gene or incorporate a designed sequence, so the same targeting system may produce different types of genetic changes. This repair step is therefore central to whether an experiment investigates gene loss or tests a planned DNA modification.
These factors determine whether an edit can reach the relevant cells, affect the intended genomic target, persist over time, and remain suitable for medical use. Improving them is essential for moving from laboratory investigation toward clinical applications. They also shape how confidently researchers can interpret disease models, engineered cell therapies, and potential treatments for inherited disorders.
The technology can be used to investigate how particular genetic changes contribute to disease, while also supporting efforts to modify those sequences. In medicine, this creates a link between understanding disease mechanisms and exploring interventions. Research may therefore focus on explaining a disorder, developing an engineered cell therapy, or investigating a treatment for an inherited condition.
A typical approach identifies a genomic sequence of interest, uses a guide RNA to direct a CRISPR-associated nuclease such as Cas9 to that matching target, and creates a DNA break there. The cell then repairs the break, either disrupting the gene or introducing a designed sequence. The resulting repair outcome provides the basis for interpreting the experiment.
Medical applications include studying disease mechanisms, developing engineered cell therapies, and investigating treatments for inherited disorders. These uses span both research and therapeutic development: disease models can help clarify genetic causes, while targeted modifications can support exploration of possible interventions. The approach is especially relevant when a disorder involves a DNA sequence that researchers aim to investigate or modify.
Inherited disorders are linked to genetic changes passed through DNA, making targeted sequence modification relevant to treatment research. CRISPR can help researchers investigate those disease-associated sequences and explore whether a gene can be disrupted or a designed sequence introduced. Its clinical significance still depends on achieving accurate editing, effective delivery, durable results, and acceptable safety.