Tissue Sampling Technique
Tissue Sampling Technique - Related Videos
03:42
Solid Phase Microextraction Sampling: A Probe-based Technique for Sample Extraction from Graft Tissues
0 Views •
2023
In this video, we demonstrate SPME-probe-based extraction of metabolites from a pre-harvested donor kidney. The extracted metabolites can be used to perform metabolomic and lipidomic analysis in order to assess the organ quality for transplantation.
08:36
Efficient Techniques for Comprehensive Sampling of Accessible Tissues in Adult Xenopus
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2025
This is part one of a comprehensive Xenopus sampling protocol. The tissues sampled are the heart ventricle, arterial trunk, left liver lobe, gallbladder, lung, pancreas, spleen, larynx, esophagus, stomach, intestines, testes, fat bodies, oviduct, paired kidneys, sciatic plexus, skin, thymus, and whole eye.
08:59
Human Subcutaneous Adipose Tissue Sampling Using a Mini-Liposuction Technique
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Cited by 5 •
2021
The manuscript and associated video demonstrate a percutaneous biopsy technique to obtain samples of subcutaneous adipose tissue from areas surrounding the umbilicus. This method is a low-risk and efficient way to investigate a range of parameters (e.g., gene or protein expression, enzyme activity, lipid content) within adipose tissue.
05:09
Mitochondrial Isolation: A Technique to Isolate Mitochondria from Human Ovarian Cancer Tissue Sample
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2023
This video describes the procedure for isolating mitochondria from human ovarian cancer tissues using differential-speed centrifugation in combination with density gradient centrifugation. The isolated mitochondria can be used for proteomic analysis of human ovarian cancer mitochondrial proteome.
04:37
Sampling Prostate Cancer Tissue for Biobanking: An MRI-guided Targeted Technique to Remove Biopsy Punches from Fresh Tumor Tissue
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2023
This video demonstrates an MRI data-based technique to target specific tumor-containing regions in prostate tissue for sampling. The collected biopsy specimens can be either stored for biobanking purposes or used for subsequent downstream analysis.
FAQs
A sample must reflect the tissue abnormality being investigated; otherwise, microscopic or molecular findings may not accurately characterize the condition. Location and sampling approach therefore influence whether the specimen contains relevant cellular changes, inflammation, infection, or abnormal growth. Selecting an appropriate portion of tissue improves the reliability of histopathological findings and supports more dependable clinical or research conclusions.
These approaches provide different ways to obtain tissue according to the suspected condition and the tissue’s location. Needle sampling accesses tissue through a needle, endoscopic sampling uses an endoscope, punch sampling removes a tissue portion with a punch, and surgical excision obtains tissue surgically. The selected approach determines how the specimen is collected before laboratory examination.
Collection is followed by fixation, processing, and sectioning so the specimen can be prepared for examination. These steps produce tissue sections suitable for microscopic analysis, allowing cellular structure and tissue changes to be assessed. Proper preparation connects the original specimen with the observations used to identify inflammation, infection, abnormal growth, or other clinically relevant changes.
Microscopic examination can reveal structural and cellular findings, including inflammation, infection, and abnormal growth. Molecular testing can add information about genetic or biochemical features. Together, these forms of analysis provide complementary evidence: histopathology describes tissue changes, while molecular results characterize selected features that may assist disease classification, treatment planning, or research interpretation.
A typical workflow begins by selecting a sampling approach suited to the suspected condition and tissue location, then obtaining the specimen with a needle, endoscope, punch, or surgical excision. The tissue is subsequently fixed, processed, and sectioned for analysis. Microscopic examination and, when appropriate, molecular testing then generate findings for clinical or research use.
The collection method determines the principal equipment: a needle, endoscope, punch, or surgical instruments may be used to obtain the specimen. After collection, laboratory preparation requires fixation, processing, and sectioning before analysis. These components support the transition from tissue acquisition to a form suitable for microscopic examination or molecular testing.
In medicine, tissue sampling supports several stages of patient care. Clinicians may use its findings for diagnosis, disease classification, treatment planning, and monitoring. The resulting evidence can come from microscopic assessment of tissue structure and cellular changes, molecular identification of genetic or biochemical features, or both, depending on the suspected condition and the examination required.
Clinical samples can provide material for examining tissue structure, composition, and molecular characteristics, while research analyses use the same categories of evidence to investigate disease-related changes. Careful selection of representative tissue improves the reliability of histopathological and research findings. This makes sampling relevant not only to individual diagnosis and monitoring but also to broader disease investigation.