Ex vivo הדמיה של תאים T ב Slices צומת Murine לימפה עם widefield ו מיקרוסקופים confocal

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Title

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Isolation and Labeling of T cells from Mouse Lymph Nodes

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Plating Labeled T cells onto Lymph Node Slices

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Preparation of Microscope and Lymph Node Slices for Imaging

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Imaging T cells with a Widefield Inverted Microscope

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Imaging T cells with a Confocal Upright Microscope

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Representative Images and Movies of T cell Accumulation, Recruitment, and Motility within Lymph Node Slices

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Conclusion