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December 30, 2016
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Summary
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פרוטוקול זה מתאר את דור phenotyping לשחזור של תאי T רגולטוריים מושרה אדם (iTregs) מתאי CD4 + T נאיביים במבחנה. פרוטוקולים שונים לזירוז FOXP3 לאפשר לחקר פנוטיפים iTreg הספציפיים שהושגו עם פרוטוקולי בהתאמה.
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Cite this Article
Schmidt, A., Éliás, S., Joshi, R. N., Tegnér, J. In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol. J. Vis. Exp. (118), e55015, doi:10.3791/55015 (2016).
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