9,312 Views
•
12:24 min
May 05, 2018
DOI:
10.3791/57471-v
פורמלין-קבוע דגימות פרפין-מוטבע מהווים מקור חשוב של סמנים מולקולריים של מחלות אנושיות. כאן אנו מציגים פרוטוקול הכנה ספריית cDNA מבוסס המעבדה, בתחילה עוצב עם RNA טריים קפואים, באופן מיטבי עבור הניתוח של מיקרו Rna בארכיון של רקמות מאוחסן עד 35 שנים.
00:09
A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
Related Videos
7295 Views
09:26
Cerebrospinal Fluid MicroRNA Profiling Using Quantitative Real Time PCR
15386 Views
13:00
A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
11711 Views
11:29
miRNA Expression Analyses in Prostate Cancer Clinical Tissues
10807 Views
12:53
Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species
26674 Views
12:21
MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
14894 Views
14:15
Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
11849 Views
11:52
Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
10316 Views
08:30
Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
50907 Views
02:00
FFPE Tissue Pretreatment for RNA CISH: A Procedure to Process Formalin-Fixed Paraffin-Embedded Tissue Sections for RNA Chromogenic In Situ Hybridization
1159 Views
Read Article
Cite this Article
Loudig, O., Liu, C., Rohan, T., Ben-Dov, I. Z. Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens. J. Vis. Exp. (135), e57471, doi:10.3791/57471 (2018).
Download .ris file
Copy
Share Video
.