הכנת כלורופלסט תאי המשנה של תודרנית לניתוח של חלבון לוקליזציה Immunoblotting או פרוטאומיקס

1:01

Harvesting of Arabidopsis Leaves

2:26

Purification of Crude Chloroplasts Using Differential Centrifugation

3:30

Purification of Intact Chloroplasts on Continuous Percoll Gradient

4:51

Lysis of Intact Chloroplasts Using a Hypotonic Buffer and Purification of Chloroplast Sub-Compartments on Discontinuous Sucrose Gradients

6:05

Washing and Concentration of Thylakoid and Envelope Membrane Systems

7:09

Results: Analysis of Protein Localization by Immunoblotting or Proteomics

8:44

Conclusion