השתלה אורתוטופית של גידולי שד כמודלים פרה-אקליניים לסרטן השד

Overview

This protocol describes the orthotopic transplantation of breast tumor fragments into the mammary fat pad, providing valuable approaches for investigating tumor biology, drug responses, and mechanisms of drug resistance. This minimally invasive technique ensures proper placement of tumor tissues and maintains animal mobility.

Key Study Components

Area of Science

• Breast cancer research
• Transplantation techniques
• Drug response evaluation

Background

• Patient-derived xenograft (PDX) models recapitulate human disease.
• Orthotopic transplantation allows for better study of tumor biology.
• Minimally invasive techniques improve animal welfare.
• Multiple therapies can be evaluated simultaneously.

Purpose of Study

• To investigate tumor biology in a relevant model.
• To evaluate drug responses in breast cancer therapies.
• To understand mechanisms of drug resistance.

Methods Used

• Orthotopic transplantation of tumor fragments.
• Minimally invasive surgical techniques.
• Monitoring of tumor placement and animal mobility.
• Evaluation of multiple candidate therapies in preclinical trials.

Main Results

• Successful placement of tumor tissues within the mammary fat pad.
• Improved accessibility for counting and measurement.
• Ability to transplant multiple animals from a single patient-derived graft.
• Enhanced evaluation of drug responses compared to subcutaneous models.

Conclusions

• The described method is superior for establishing patient-derived models.
• It allows for comprehensive studies of tumor biology and therapy responses.
• Visualization techniques aid in ensuring successful procedures.

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