השתלה והערכה של מלנומה בכורואיד מורין באמצעות טומוגרפיה קוהרנטית אופטית

Overview

This protocol describes a method for implanting and evaluating melanoma in the murine choroid using optical coherence tomography (OCT). It enables the induction of reproducible choroidal melanoma tumors in mice and allows for live imaging of tumor growth.

Key Study Components

Area of Science

• Neuroscience
• Oncology
• Imaging Techniques

Background

• Establishing tumors in the posterior part of the eye is challenging.
• Uveal melanoma has a high rate of metastasis, making research critical.
• Experimental models are essential for developing new therapeutic options.
• Live imaging allows for continuous monitoring of tumor growth.

Purpose of Study

• To develop a reliable method for inducing choroidal melanoma in mice.
• To utilize OCT for real-time assessment of tumor location and growth.
• To create homogenous study groups for better experimental outcomes.

Methods Used

• Injection of tumor cells into the murine choroid.
• Utilization of optical coherence tomography for live imaging.
• Assessment of tumor growth in live animals.
• Evaluation of the success of injection immediately post-procedure.

Main Results

• Successful induction of reproducible choroidal melanoma tumors.
• Effective monitoring of tumor growth through live imaging.
• Ability to assess tumor location at the time of inoculation.
• Creation of study groups with homogenous tumors for research.

Conclusions

• This method provides a valuable tool for studying choroidal melanoma.
• Live imaging enhances the ability to monitor tumor progression.
• It contributes to the advancement of therapeutic research in uveal melanoma.

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