גישות בביולוגיה מבנית וכימיה אנליטית לאפיון אנזימים מטבוליים של C-גליקוזיד במיקרוביוטה של המעי האנושי

Construction of Expression Vector

Purification of Protein

Circular Dichroism (CD) Spectrum Data Collection

Protein Crystal Growth and Optimization

Data Collection and Structure Determination of Protein Crystals

Monitoring of DgpA/B/C Activity by HPLC

Monitoring of DgpA Activity with 2,6-dichlorophenol indophenol (DCPIP) Assay

Determination of Inhibition Rate of Glucose on the C-glycosidic Bond Cleavage Reaction of Puerarin

Determination of Kinetic Parameters

Preparation of Reaction Intermediate Product

Characterization of Reaction Intermediate Product with LC-MS

Characterization of Reaction Intermediate Product with NMR

Representative Results

Conclusion