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HIGH SCHOOL

Chemistry

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Chemistry

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Analytical Chemistry

Ion-Exchange Chromatography for Sample Cleanup

Description

Ion-exchange chromatography is a separation method that cleans up samples by using charge. A column is packed with an ion-exchange resin, which is a charged stationary phase on a solid support. Strong cation-exchange chromatography uses a negatively charged resin to separate cations. Strong anion-exchange chromatography uses...

Transcript

La cromatografia a scambio ionico è ampiamente utilizzata nella separazione e nell'isolamento di composti carichi, in particolare di biomolecole di grandi dimensioni.

Questo tipo di cromatografia liquida utilizza una colonna di perle impaccate in fase stazionaria, chiamate resina. La tecnica separa gli analiti in base alla loro affinità c...

Tags

Cation Exchange ChromatographyAnion Exchange ChromatographyProtein SeparationSalt Gradient ElutionColumn EquilibrationBuffer pH SelectionHydroxyapatite ChromatographyImmobilized Metal Affinity ChromatographyHigh Performance Liquid Chromatography

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