Name: spindle position and cell division plane
Uploaded: 2023-04-30T18:28:51.000Z
Duration: 2 min 13 s
Channel: JoVE

K12 Standards

Pennsylvania State Academic Standards

9-12

Life Science

Inheritance and Variation of Traits

3.1.9-12.D

Determining the Plane of Cell Division

Una cellula in divisione che determina con precisione il suo piano di divisione cellulare è essenziale per la separazione di successo dei cromosomi e la distribuzione del contenuto citoplasmatico alle sue cellule figlie.
Negli animali, il piano di divisione si trova all'equatore della cellula tra i cromosomi segregati e la sua posizione viene determinata durante l'anafase. I microtubuli che formano il fuso mitotico interagiscono con la corteccia cellulare e svolgono un ruolo decisivo nello specificare il solco di scissione.
Per spiegare la determinazione del piano di divisione cellulare vengono utilizzati tre diversi modelli: il modello di stimolazione astrale, il modello di rilassamento polare e il modello di stimolazione del fuso centrale.
Nel modello di stimolazione astrale, schiere radiali di microtubuli astrali interagiscono con la corteccia equatoriale e inducono la formazione del solco di scissione.
Nel modello di rilassamento polare, i microtubuli astrali inviano segnali inibitori ai poli cellulari e quindi sopprimono la formazione del solco di scissione su entrambi i poli. Questo porta alla contrazione corticale all'equatore cellulare.
Una volta iniziata l'anafase, i microtubuli interpolari presenti tra i cromosomi segregati si riorganizzano nei microtubuli antiparalleli sovrapposti, noti anche come fuso centrale. Nel modello di stimolazione del fuso centrale, il fuso centrale stimola la formazione del solco di scissione nella corteccia equatoriale. 
Il consenso generale è che tutti e tre i processi agiscono insieme per determinare il piano di divisione cellulare. Questo approccio su più fronti può far sì che il sistema risponda alle fluttuazioni della concentrazione proteica e delle dimensioni delle cellule, aumentando così l'accuratezza della divisione cellulare.
Il contributo di ciascun metodo dipende dal tipo e dalle dimensioni della cellula. Ad esempio, negli embrioni di C. elegans, è presente una vasta gamma di microtubuli astrali e quindi può svolgere un ruolo più attivo nella determinazione del piano di divisione cellulare.

<ol>
	<li>Biologia molecolare della cellula, Alberts, 6a edizione. Pagine 997-999.</li>
	<li>Biologia cellulare molecolare, Lodish, 8a edizione. Pagine - 903.</li>
	<li>Geelen, Danny. "Il posizionamento del piano di divisione è determinato da un sito di divisione corticale sulla membrana plasmatica." In Biotecnologie e Agricoltura Sostenibile 2006 e oltre, pp. 121-124. Springer, Dordrecht, 2007.</li>
	<li>Balasubramanian, Mohan K., Erfei Bi e Michael Glotzer. "Analisi comparativa della citocinesi nel lievito in gemmazione, nel lievito di fissione e nelle cellule animali". Current Biology 14, n. 18 (2004): R806-R818.</li>
	<li>Chant, John. "Scaffold di septin e piani di scissione in Saccharomyces." Cella 84, n. 2 (1996): 187-190.</li>
	<li>Rincon, Sergio A. e Anne Paoletti. "Mid1/anillina e la regolazione spaziale della citochinesi nel lievito di fissione." Citoscheletro 69, n. 10 (2012): 764-777.</li>
	<li>Galli, Matilde e Sander van den Heuvel. "Determinazione del piano di scissione nei primi embrioni di C. elegans". Rassegna annuale di genetica 42 (2008): 389-411.</li>
</ol>

determinazione del piano di divisione cellulare

Il posizionamento del piano di divisione cellulare è un passaggio fondamentale durante lo sviluppo e la differenziazione cellulare, in particolare durante la mitosi, quando il piano è essenziale per determinare la dimensione delle due cellule figlie. Il piano di divisione cellulare è perpendicolare al piano di segregazione cromosomica, ma diversi tipi di organismi hanno meccanismi di divisione cellulare diversi per adattarsi alla loro morfologia e funzione.

Cellule animali

Nelle cellule animali il solco di scissione si forma lungo il piano di divisione cellulare a partire dalla corteccia cellulare, la regione al di sotto della membrana plasmatica. Il fuso mitotico gioca un ruolo decisivo nel determinare il piano di divisione cellulare; pertanto, un cambiamento nell'asse del fuso mitotico può alterare il piano di divisione. Questa strategia viene utilizzata da alcuni organismi eucarioti, come Caenorhabditis elegans, per passare dalla divisione cellulare simmetrica a quella asimmetrica. La divisione cellulare simmetrica produce due cellule figlie identiche e viene spesso utilizzata per far crescere tessuti contenenti molti dello stesso tipo cellulare. Al contrario, la divisione asimmetrica crea diversità cellulare poiché genera due cellule figlie non identiche. Ad esempio, le cellule neuroepiteliali inizialmente aumentano il loro numero subendo divisioni simmetriche. Successivamente, utilizzano la divisione asimmetrica per produrre cellule figlie simili alla cellula madre e differenziare i neuroni.

Lievito

Nel lievito in erba, come Saccharomyces cerevisiae, il piano di divisione cellulare è deciso nella fase G1 dalle proteine septina. La citocinesi si ottiene formando un solco di scissione nella regione del collo delle cellule di lievito in erba; tuttavia, l'apparato mitotico non gioca alcun ruolo nel determinare il piano di divisione cellulare. Al contrario, nel lievito a fissione, come lo Schizosaccharomyces pombe, la posizione del nucleo interfase decide la posizione del piano di divisione cellulare nella fase G2. Durante l'interfase, la proteina Mid1 è localizzata principalmente nel nucleo; tuttavia, durante la mitosi, Mid1 viene esportato dal nucleo alla corteccia cellulare, dove recluta componenti dell'anello di actomiosina nella regione mediale della cellula. Ciò alla fine porta alla divisione della cellula madre in due cellule figlie di dimensioni simili.

Impianti

Nella maggior parte delle cellule vegetali, il piano di divisione cellulare viene deciso prima che la cellula inizi la mitosi. Lungo l'equatore della cellula madre si forma una fascia preprofase, che segna il piano di divisione cellulare. La banda preprofase è una struttura ad anello composta da microtubuli e F-actina. Dà origine a un fascio di microtubuli chiamato fragmoplasto, che fornisce un'impalcatura strutturale per l'espansione e il posizionamento della piastra cellulare.

Determinazione del piano di divisione cellulare

Il posizionamento del piano di divisione cellulare è un passaggio critico durante lo sviluppo e la differenziazione cellulare, in particolare durante la mitosi quando il piano è ...

Education

Didattica

JoVE Core

Cell Biology

Biologia cellulare

Unit 1

Unità 1

Cell Division

Divisione cellulare

KEY TERMS AND CONCEPTS

TERMINI E CONCETTI CHIAVE

spindle position and cell division plane

The plane of cell division is set by the position of the spindle during mitosis. Cells use this internal structure to help decide where the cell will split. The result affects how the two daughter cells are arranged after division.

This process is important in many developing tissues. It helps explain how cells divide in a controlled direction. A clear plane of division supports proper cell organization as new cells form.

The transcript focuses on how the spindle is used to determine that plane. By tracking spindle position, scientists can study how division is oriented inside the cell. This makes the concept easier to connect to the steps of mitosis and cell structure.

Spindle Position and Cell Division Plane

mitosis and cytokinesis

Stages of Cell Division in Eukaryotes

Cell division in eukaryotes follows a tightly regulated cycle that includes cell growth, DNA replication, chromosome duplication, chromosome distribution to daughter cells, and cell division. Regulatory systems and extracellular signals help control when cells divide. In typical human cells, the cycle takes about 24 hours. The cycle has two major stages. DNA is copied during the S phase of interphase. The mitotic, or M, phase then separates the duplicated chromosomes into two new nuclei and...

chromosome duplication

Chromosome Copying in Cell Division

Chromosome copying is an important step in cell division. Before a cell divides, it must make an exact copy of its chromosomes so each new cell can receive the same genetic information. A chromosome is a DNA-containing structure that carries genes. During duplication, the chromosome is copied into two identical sister chromatids. These copies stay attached until the cell is ready to separate them. This process helps ensure that daughter cells inherit a complete set of chromosomes. Accurate...

cohesins

Cohesins in Sister Chromatid Separation

Cohesins are protein complexes that help hold sister chromatids together after DNA is copied. They play a key role in cell division by keeping the two copies of a chromosome connected until the right time. These proteins act like a link between the sister chromatids. When the cell is ready to separate them, cohesins must be removed or released so each new cell can get one complete set of chromosomes. Cohesins are an important part of chromosome behavior during mitosis and meiosis. Their...

condensins

Condensin Proteins in Chromosome Packing

Condensin proteins help pack DNA into condensed chromosomes during cell division. They are part of the larger set of proteins that organize genetic material so it can be separated correctly when a cell divides. These proteins act by shaping and tightening chromosome structure. This process is important because DNA must be compacted before it can move through the stages of division. Condensins are therefore closely linked to chromosome condensation, the step that makes chromosomes more compact...

the mitotic spindle

How the Mitotic Spindle Guides Cell Division

The mitotic spindle guides chromosome movement during cell division. It is a structure made of microtubules, which are thin protein fibers in the cell. These fibers organize and help separate the genetic material before the cell splits. The spindle forms as the cell prepares to divide. Its fibers attach to chromosomes and line them up so they can be moved apart in an orderly way. This careful organization helps each new cell receive the correct set of chromosomes. The mitotic spindle is a key...

centrosome duplication

Centrosome Duplication in Cell Division

Centrosome duplication is an important step in cell division. The centrosome helps organize the cell’s internal structure, and it must be copied before a cell divides.

spindle assembly

Spindle Formation in Cell Division

Spindle formation in cell division is the process that builds the microtubule-based spindle needed to separate chromosomes. Microtubules are long protein fibers that help move and organize cell parts during division. During this process, microtubules grow and are arranged into a spindle structure around the chromosomes. The spindle forms in a way that supports chromosome movement to opposite sides of the cell. This step is essential for accurate cell division. Spindle formation depends on the...

attachment of sister chromatids

Sister Chromatid Attachment in Mitosis

Sister chromatid attachment in mitosis helps align duplicated chromosomes before they separate. Sister chromatids are the matching copies made after DNA replication. They stay connected until the cell is ready to divide. The attachment step is closely tied to the metaphase plate, the middle of the cell where chromosomes line up. This alignment helps each new cell receive one copy of every chromosome. Proper attachment is an important part of accurate cell division.

forces acting on chromosomes

Chromosome Movement During Cell Division

Chromosome movement during cell division depends on forces that act on the chromosomes. These forces help move the chromosomes into the right position as the cell divides. The transcript focuses on how these forces affect chromosome behavior during division. It highlights the role of the mitotic spindle, which is the structure that helps organize and move chromosomes. The spindle uses force to guide chromosome movement at the right time. Understanding these forces helps explain how...

separation of sister chromatids

Chromatid Pulling in Cell Division

Separation of sister chromatids is a key step in cell division. Sister chromatids are two identical copies of a chromosome that stay attached until the cell is ready to split them apart. Their separation helps each new cell receive the correct genetic material. During this stage, the sister chromatids are pulled to opposite sides of the cell. This movement happens as the cell prepares to divide into two daughter cells. Once the chromatids separate, each side of the cell has a complete set of...

the spindle assembly checkpoint

How Cells Verify Chromosome Spindle Attachment

The spindle assembly checkpoint helps cells verify that chromosomes are attached to the spindle before division continues. It acts as a safety step during cell division and helps prevent errors in chromosome separation. This checkpoint is controlled by kinetochores, which are protein structures on chromosomes that connect to spindle fibers. If a chromosome is not attached correctly, the checkpoint can delay progression until the problem is fixed. Once the attachment is correct, the cell can...

anaphase a and b

Chromosome and Spindle Movement in Anaphase

Anaphase is the stage of cell division when chromosomes move apart. In this phase, sister chromatids separate and travel toward opposite poles of the cell. Chromosome movement in anaphase can happen in two ways. In anaphase A, chromosomes move closer to the poles as the microtubules attached to them shorten. In anaphase B, the spindle poles themselves move farther apart and help stretch the cell. These two movements work together to ensure that the separated chromosomes are pulled to opposite...

anaphase promoting complex

Cell Cycle Control by APC

The anaphase-promoting complex, or APC, helps control cell cycle progression. It acts during cell division to help move the process from one stage to the next. This control is important for proper timing in the cell cycle. APC is a protein complex that works as a regulator. It helps trigger key steps that allow division to continue in an ordered way. By guiding this transition, APC supports normal cell cycle control.

the contractile ring

Cell Division and the Contractile Ring

The contractile ring helps cells divide at the end of cell division. It forms at the cell's center and tightens to split one cell into two. This process is part of cytokinesis, the stage when the cytoplasm divides. The ring is made of actin and myosin, two proteins that can work together to create force. As the ring contracts, it draws the cell membrane inward. This pinch helps separate the two daughter cells. Understanding the contractile ring is important for studying how cells complete...

determining the plane of cell division

The plane of cell division is set by the position of the spindle during mitosis. Cells use this internal structure to help decide where the cell will split. The result affects how the two daughter cells are arranged after division. This process is important in many developing tissues. It helps explain how cells divide in a controlled direction. A clear plane of division supports proper cell organization as new cells form. The transcript focuses on how the spindle is used to determine that...

the phragmoplast

Phragmoplast in Plant Cell Division

The phragmoplast is a plant cell structure that forms during cell division. It helps guide the final stage of division, when the cell begins to split into two daughter cells. In plant cells, the phragmoplast appears between the two new nuclei. It is part of the process that leads to the formation of a new cell wall. This wall separates the daughter cells after division is complete. The phragmoplast plays an important role in plant cytokinesis, which is the physical division of the cytoplasm.

distribution of cytoplasmic content

Cytoplasmic Content During Cell Division

Cytoplasmic content is distributed as a cell divides. This process helps each new cell receive the materials it needs to function. The cytoplasm includes the fluid and structures inside the cell membrane, but outside the nucleus. During cell division, this content is separated between the two daughter cells. The division must be organized so both cells can continue normal activity after the split. The video focuses on how this distribution occurs as part of cell division. It highlights the...

key terms and concepts

Learn how spindle position helps determine the plane of cell division during mitosis and why this matters for daughter cell arrangement in developing tissues.

Spindle Position and Cell Division Plane | JoVE K12

cell biology

JoVE Core Cell Biology illustrates complex cellular processes in easy-to-understand yet comprehensive animated video lessons. This collection focuses on the essential components and functions of cells, including organelles and membranes, cellular transport, and cell energetics. It is complemented by scientist-in-action videos that demonstrate the application of cell biology in real-world research experiments.

Louisiana Student Standards for Science

High School

Life Sciences

from-molecules-to-organisms-structures-and-processes

From Molecules To Organisms: Structures And Processes

HS-LS1-4

Tennessee Academic Standards

Biology

From Molecules to Organisms: Structures and Processes

BIO1.LS1.6

Minnesota Academic Standards in Science

3.1.1 9L.3.1.1.2

new-jersey-student-learning-standards-for-science

New Jersey Student Learning Standards for Science

Arizona Science Standards

HS+B.L1

HS+B.L1U1.9

Alabama Course of Study Science

9-12.B.1.3

Florida Department of Education

SC.912.L.16

SC.912.L.16.14

south-carolina-college-and-career-ready-science-standards

South Carolina College and Career Ready Science Standards

B-LS1-4.

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Positive Regulator Molecules

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Cohesins

Condensins

The Mitotic Spindle

Centrosome Duplication

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Attachment of Sister Chromatids

Forces Acting on Chromosomes

Separation of Sister Chromatids

The Spindle Assembly Checkpoint

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Anaphase Promoting Complex

The Contractile Ring

The Phragmoplast

Distribution of Cytoplasmic Content

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DNA Packaging

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3.1.9-12.P

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3.1.9-12.Q

Spindle Position and Cell Division Plane

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