Question 1

What is a transgene and how does it differ from an organism's native genes?

Accepted Answer

A transgene is a foreign gene, typically from another species, inserted into an organism's genome to produce a desired protein. Unlike native genes, transgenes are artificially introduced through genetic engineering techniques like recombinant DNA and DNA cloning. For example, transgenic goats produce spider silk in their milk by expressing an inserted transgene that doesn't naturally occur in goats.

Question 2

Why is a transgene introduced at the fertilized egg stage in animals?

Accepted Answer

Introducing the transgene at the fertilized egg stage ensures it integrates into germ cells, the reproductive precursor cells that become gametes. This germline integration allows the transgene to be present in all developing cells and be inherited through breeding, creating stable lines of transgenic organisms that pass the foreign gene to offspring.

Question 3

What methods are used to introduce transgenes into plant cells?

Accepted Answer

Plants commonly use biological methods, particularly bacterial vector delivery. Rhizobium radiobacter, a soil bacterium, naturally infects plants and integrates its plasmid DNA into the plant genome. Scientists modify this bacterium to carry transgenes, then culture plant tissue samples with the bacterium to allow infection and transgene integration into the plant's genome.

Question 4

How do scientists identify cells that have successfully integrated a transgene?

Accepted Answer

Scientists include selectable markers, such as antibiotic resistance genes, or reporter genes like GFP alongside the gene of interest. These markers allow researchers to identify and select cells where the transgene has successfully integrated into the genome. Cells without successful integration lack these markers and can be eliminated during selection.

Question 5

What are the main practical applications of transgenic organisms?

Accepted Answer

Transgenic organisms have diverse applications across agriculture, medicine, and industry. Transgenic plants provide insect resistance to reduce pesticide use; bacteria produce biofuels and human proteins for medicine; and transgenic animals serve as models for human disease research and manufacture therapeutic proteins, advancing both scientific understanding and practical solutions.

Question 6

What is the difference between stable and transient transgene expression?

Accepted Answer

Stable transgene expression occurs when the transgene integrates into the organism's genome, allowing permanent, heritable expression across generations. Transient expression happens when the transgene remains in the cell but doesn't integrate, resulting in temporary protein production that isn't passed to offspring or maintained long-term.

Question 7

How do scientists create homozygous transgenic animal lines?

Accepted Answer

Scientists use backcrossing, a process where transgenic offspring are mated with their parent to concentrate the transgene. Repeated backcrossing produces lines where both copies of the target gene carry the transgene, creating homozygous transgenic animals that consistently express the desired trait across all offspring.

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